LGB ELISA Kit (Bovine)
Cat# OKEH03893
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
LGB ELISA Kit (Bovine) (OKEH03893)
| Datasheets/Manuals | Printable datasheet for LGB ELISA Kit (Bovine) (OKEH03893) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology LGB ELISA Kit (Bovine) (OKEH03893) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LGB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LGB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LGB captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 1.56-100ug/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Average recovery when spiking into serum: 106% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay CV: <6.1% (n=20) Inter-Assay CV: <9.9% (n=24) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.47ug/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Beta-lactoglobulin | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | PAEP |
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| Gene Full Name | progestagen-associated endometrial protein |
| Alias Symbols | beta lactoglobulin C, beta lactoglobulin D, beta-lactoglobulin, beta-LG, BLG, bLG C, lactoglobulin, beta, LGB. |
| NCBI Gene Id | 280838 |
| Protein Name | Beta-lactoglobulin |
| Description of Target | Primary component of whey, it binds retinol and is probably involved in the transport of that molecule. |
| Uniprot ID | P02754 |
| Protein Accession # | NP_776354.2 |
| Nucleotide Accession # | NM_173929.3 |
| Protein Size (# AA) | 178 |
| Molecular Weight | 20 kDa |
