This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTE4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTE4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Reactivity
All
Range
31.25-2000 pg/mL
Sensitivity
9.41 pg/mL
Protein Length
2h
Alternative names
Cysteinyl Leukotrienes E4
Note
For research use only
Sample Types
serum, plasma, tissue homogenates, cell culture supernates and other biological fluids