M-CSF/CSF1 Protein, Human, Recombinant V2
Cat# TMPY-00127U-20ug
Size : 20ug
Brand : TargetMol
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M-CSF/CSF1 Protein, Human, Recombinant V2
(Synonyms: M-CSF, MCSF, CSF-1, colony stimulating factor 1 (macrophage)) Copy Product InfoM-CSF/CSF1 Protein, Human, Recombinant V2 is expressed in HEK293 Cells. The accession number is P09603-1.
For research use only—not for human use. No sales to individuals. Use as intended only.
User Guide of Recombinant Proteins
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human M-CSF at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 42.8 ng/ml determined by ELISA (QC Test). Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200). Measured in a cell proliferation assay using RAW264.7 cells.The ED50 for this effect is 2.5 - 7.0 ng/mL (QC Test). |
| Description | M-CSF/CSF1 Protein, Human, Recombinant V2 is expressed in HEK293 Cells. The accession number is P09603-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | Tag Free |
| Accession Number | P09603-1 |
| Construction | Glu33-Asn190 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | M-CSF, MCSF, CSF-1, colony stimulating factor 1 (macrophage) |
| Research Background | CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. |
| Molecular Weight | 20.5 kDa (Predicted); 23-28 kDa (Due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |