M2, Recombinant, Influenza A Virus, aa1-22, His-Tag (Matrix Protein 2)

Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation.

Partial recombinant protein corresponding to aa1-22 from infuenza A virus M2 (strain A/USA:Phila/1935 H1N1), fused to 6X His-Tag at N-terminal expressed in E. coli. Swiss/Uniprot Accession: A4GCM0

Molecular Weight:
~6.5kD

Amino Acid Sequence:
MSLLTEVETPIRNEWGCRCNGS

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.