Reagents and instruments for immunology, cell biology and molecular biology.
 
> MAP2a,b,c (BSA & Azide Free) (Microtubule Associated Protein 2a,b,c) (Biotin)

MAP2a,b,c (BSA & Azide Free) (Microtubule Associated Protein 2a,b,c) (Biotin)

Print Print
Please log in

Cat# M2350-01BX-Biotin-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

M2350-01BX-Biotin MAP2a,b,c (BSA & Azide Free) (Microtubule Associated Protein 2a,b,c) (Biotin)

Clone Type
Monoclonal
Host
mouse
Source
bovine
Isotype
IgG1
Grade
Affinity Purified
Applications
IF IHC IP WB
Crossreactivity
Bo Hu Rt Xe
Shipping Temp
Blue Ice
Storage Temp
-20°C

The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
IMR-5 or T98G human glioblastoma cells or brain.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 0.N.395|Host: mouse|Source: bovine|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.|Purity: Purified by Protein G affinity chromatography from ascites.|Immunogen: Bovine brain MAP2. Cellular Localization: Cytoplasmic. |Epitope: aa1-151.|Specificity: Recognizes the phosphorylated form of bovine MAP2a,b,c and shows no crossreactivity with other MAPs, tau and tubulin. Recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). Reacts with dendrites and cell bodies of neurons. Species Crossreactivity: human, rat, Xenopus and quail. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Bovine brain MAP2. Cellular Localization: Cytoplasmic. |Epitope: aa1-151.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes the phosphorylated form of bovine MAP2a,b,c and shows no crossreactivity with other MAPs, tau and tubulin. Recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). Reacts with dendrites and cell bodies of neurons. Species Crossreactivity: human, rat, Xenopus and quail.
References
1. Kalcheva N; et al. Journal of Neurochemistry, 1994 Dec, 63(6):2336-41.2. Tucker RP; et al. Journal of Neuroscience, 1988 Dec, 8(12):4503-12.3. Viereck C; et al. Neuroscience, 1988 Sep, 26(3):893-904.