Measles IgG ELISA Kit

Intended Uses: Leukemia MLL-AF4 Fusion Gene real time RT-PCR Kit is used for the detection of Leukemia MLL-AF4 Fusion Gene in leukocyte by using real time PCR systems.

Principle of the Assay: The principle of the real-time detection is based on the fluorogenic 5'nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5' end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real-time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.

Small volumes of Measles IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS580113 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Measles IgG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Measles IgG. The ELISA analytical biochemical technique of the MBS580113 kit is based on Measles IgG antibody-Measles IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Measles IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Measles IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).