MHC Class 1 (MHC Class I) (APC)

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Cat# M3885-48Q-APC-100ul

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

M3885-48Q-APCMHC Class 1 (MHC Class I) (APC)

Specifications

Clone TypeMonoclonal

Hostmouse

Sourcemouse

IsotypeIgG2b

GradeAffinity Purified

ApplicationsFC IP

CrossreactivityBo Gp Hm Hu Mo Po Rt Sh

Shipping TempBlue Ice

Storage Temp4°C Do Not Freeze

MHC Class 1 is expressed at varying levels on the majority of nucleated cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.

Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63 myeloma cells with spleen cells from C1D mice.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG2b|Clone No: 10F80 (2G5)|Host: mouse|Source: mouse|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Purified H-2Kb and H-2Db MHC-I molecules.|Specificity: Recognizes the monomorphic epitope present on mouse MHC Class 1. The epitope recognised is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this may recognize a polymorphic epitope of canine MHC class I. Species Crossreactivity: rat, guinea pig, sheep, bovine, porcine, human, hamster. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Purified H-2Kb and H-2Db MHC-I molecules.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Purity

Purified by Protein A affinity chromatography from tissue culture supernatant.

Specificity

Recognizes the monomorphic epitope present on mouse MHC Class 1. The epitope recognised is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this may recognize a polymorphic epitope of canine MHC class I. Species Crossreactivity: rat, guinea pig, sheep, bovine, porcine, human, hamster.