MMAF [745017-94-1]
Cat# T3256-5mg
Size : 5mg
Brand : TargetMol
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Phone : +1 850 650 7790
MMAF
(Synonyms: MonoMethyl auristatin F) Copy Product Info
MMAF (MonoMethyl auristatin F), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin.
Cas No. 745017-94-1
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:99.88%
Appearance:Solid
Color:White
COA LCMS HNMR
Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | MMAF (MonoMethyl auristatin F), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin. |
| Targets & IC50 | H3397 cells:105 nM. |
| In vitro | MMAF shows in vitro cytotoxicity against a panel of cell lines. The IC50 values for Karpas 299, H3396, 786-O and Caki-1 are 119, 105, 257, and 200 nM, respectively. Targeted MMAF is much more potent than the free drug, and that cAC10 conjugates of MMAF display pronounced activities. On a molar basis, the cAC10-L1-MMAF4 is an average of over 2200-fold more potent than free MMAF and is active on all the CD30-positive cell lines tested[1]. |
| In vivo | The maximum tolerated dose in mice of MMAF (>16 mg/kg) is much higher than MMAE (1 mg/kg). cAC10-L1-MMAF4 has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF4 ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively[1]. |
| Synonyms | MonoMethyl auristatin F |
| Cell Research | Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay[2]. |
| Molecular Weight | 731.962 |
| Formula | C39H65N5O8 |
| Cas No. | 745017-94-1 |
| Smiles | O=C(O)[C@@H](NC([C@H](C)[C@H]([C@H]1N(C(C[C@@H](OC)[C@@H](N(C)C([C@@H](NC([C@@H](NC)C(C)C)=O)C(C)C)=O)[C@@H](C)CC)=O)CCC1)OC)=O)CC2=CC=CC=C2 |
| Relative Density. | 1.116 g/cm3 (Predicted) |
| Storage | Keep away from moisture,The compound is unstable in solution. Please use soon, Powder: -20°C for 3 years Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 250.00 mg/mL (341.55 mM), Sonication is recommended.  | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
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Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Product Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Monomethylauristatin FMMAFMicrotubuleAssociatedMicrotubule/TubulinMicrotubule AssociatedInhibitorinhibitCytotoxicity( Karpas 299 cell)ADCCytotoxinADC PayloadADC Cytotoxin
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Related Tags: MMAF chemical structure | MMAF in vivo | MMAF in vitro | MMAF formula | MMAF molecular weight
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