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Monkeypox Virus (MPXV) D6L ELISA Kit
Monkeypox Virus (MPXV) D6L ELISA Kit
The RayBiotech Monkeypox Virus (MPXV) D6L ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of MPXV protein D6L in serum, plasma, and cell culture supernatants.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Monkeypox virus (MPXV) |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Accession Number | Q8V570 |
| Gene Id | 929030 |
| Gene Symbols | D6L |
| Protein Name / Synonyms | D6L |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, Mpox |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Note | This ELISA uses an antibody pair and standard that have been validated for multiplex array products, but not for the 96-well plate ELISA format. Thus, this ELISA requires a 4 - 7 week development phase, which RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests, and will thereafter have a typical 1 - 2 day lead time for orders placed. |
Introduction
The RayBio® Monkeypox Virus (MPXV) D6L ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of MPXV D6L in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for MPXV D6L coated on a 96-well plate. Standards and samples are pipetted into the wells and MPXV D6L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-MPXV D6L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPXV D6L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Microplate reader capable of measuring absorbance at 450nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 µL to 1 µL volumes
- Adjustable 1-25 µL pipettes for reagent preparation
- 100 µL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard and sample dilutions
- Distilled or deionized water
- Absorbent paper
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µL of each standard and sample to each well.
- Incubate 2.5 hours at room temperature or overnight at 4ºC with gentle shaking.
- Add 100 µL prepared detection antibody to each well.
- Incubate 1 hour at room temperature with gentle shaking.
- Add 100 µL prepared HRP-Streptavidin solution.
- Incubate 1 hour at room temperature with gentle shaking.
- Add 100 µL TMB One-Step Substrate Reagent to each well.
- Incubate 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Monkeypox Virus (MPXV) D6L ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified D6L, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development