Monoclonal Mouse Anti-Human IgM Secondary Antibody (clone MuHC2, IHC, IF, WB)
Cat# LS-C391781-20
Size : 20ug
Brand : LSBio
Contact local distributor :
Phone : +1 850 650 7790
Overview
Antibody:
Human IgM Mouse anti-Human Monoclonal (MuHC2) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Ready-to-Use Azide-free, BSA-free
Specifications
Description
Human IgM antibody LS-C391781 is an unconjugated mouse monoclonal antibody. Validated for IF, IHC and WB.
Target
Human IgM
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
MuHC2
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.
Immunogen
Human Mu heavy chain.
Specificity
Human IgM
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (0.5 - 1 µg/ml)
- Western blot (0.5 - 1 µg/ml)
Usage
IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body. Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
1X PBS, pH 7.4, 0.1 mg/ml BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@biovalley.fr