RayBio® C-Series Mouse Angiogenesis Antibody Array 1 Kit. Detects 24 Mouse Angiogenic Factors. Suitable for all liquid sample types.
Mouse Angiogenesis Array C1
Mouse Angiogenesis Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 24 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| Fas Ligand (TNFSF6)
| bFGF
| GCSF
| GM-CSF
|
IFN-gamma
| IGF-2
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-12 p40/p70
|
IL-12 p70
| IL-13
| IL-6
| IL-9
| Leptin
|
MCP-1 (CCL2)
| M-CSF
| MIG (CXCL9)
| Platelet Factor 4 (CXCL4)
| TIMP-1
|
TIMP-2
| TNF alpha
| Thrombopoietin (TPO)
| VEGF-A
| |
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Angiogenesis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Loss of the tumor suppressor BTG3 drives a pro-angiogenic tumor microenvironment through HIF-1 activation
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2020
- Journal Name:
- Cell death and disease
- PubMed ID:
- 33311481
Induced Pluripotent Stem Cells Attenuate Endothelial Leakage in Acute Lung Injury via Tissue Inhibitor of Metalloproteinases-1 to Reduce Focal Adhesion Kinase Activity
- Species:
- Mouse
- Sample Type:
- Conditioned media (cell culture media, murin-induced pluripotent stem cells (miPSCs))
- Year:
- 2019
- Journal Name:
- Stem Cells
The prednisolone phosphateinduced suppression of the angiogenic function of tumorassociated macrophages enhances the antitumor effects of doxorubicin on B16.F10 murine melanoma cells in vitro
- Species:
- Human
- Sample Type:
- Cell Lysate (Cell co-culture)
- Year:
- 2019
- Journal Name:
- Oncology Reports
Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Bone Marrow fibroblasts and MKN28 gastric cancer cell lines)
- Year:
- 2016
- Journal Name:
- Oncogene
Altered expression of platelet factor 4 and basic fibroblast growth factor correlates with the inhibition of tumor growth in mice
- Species:
- Mouse
- Sample Type:
- Plasma (Taxol and control treated mice)
- Year:
- 2015
- Journal Name:
- Biomedicine and Pharmacotherapy
Liposomal simvastatin inhibits tumor growth via targeting tumor-associated macrophages-mediated oxidative stress
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Tumor lysates from B16 melanoma)
- Year:
- 2014
- Journal Name:
- Cancer Letters
- PubMed ID:
- 25444912
Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential
- Species:
- Mouse
- Sample Type:
- Conditioned Media (pigemented and nonpigmented melanocytes)
- Year:
- 2014
- PubMed ID:
- 25406462
Chemopreventive Effect of Korean Angelica Root Extract on TRAMP Carcinogenesis and Integrative “Omic†Profiling of Affected Neuroendocrine Carcinomas
- Species:
- Mouse
- Sample Type:
- Cell Lysate (Neruoendocrine Carcinoma)
- Year:
- 2014
Tumor angiogenesis is caused by single melanoma cells in a manner dependent on reactive oxygen species and NF-B.
- Species:
- Mouse
- Sample Type:
- Conditioned Media (mouse melanocytes)
- Year:
- 2013
- PubMed ID:
- 23843609
Intestinal and plasma VEGF levels in cirrhosis: the role of portal pressure
- Species:
- Rat
- Sample Type:
- Plasma (Cirrhotic and non-ascititc rat model)
- Year:
- 2012
- Journal Name:
- Journal of Cellular and Molecular Medicine
- PubMed ID:
- 21801303