mouse Anti-CD1a (CD1, CD1A Antigen, Cortical Thymocyte Antigen CD1A, Epidermal Dendritic Cell Marker CD1a, FCB 6, HTA1, T Cell Surface Antigen Leu 6, Cell Surface Antigen T6, T Cell Surface Antigen T6/Leu 6, T Cell Surface Glycoprotein CD1A, T6, Thymocyte Antigen CD1A) (MaxLight 550) Monoclonal Antibody [Clone:3H643 (NA1/34-HLK)]

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Cat# C2252-01W-ML550-100ul

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

C2252-01W-ML550 CD1a (CD1, CD1A Antigen, Cortical Thymocyte Antigen CD1A, Epidermal Dendritic Cell Marker CD1a, FCB 6, HTA1, T Cell Surface Antigen Leu 6, Cell Surface Antigen T6, T Cell Surface Antigen T6/Leu 6, T Cell Surface Glycoprotein CD1A, T6, Thymocyte Antigen CD1A) (MaxLight 550)

Clone Type

Monoclonal

Host

mouse

Source

human

Swiss Prot

P06126

Isotype

IgG2a

Grade

Highly Purified

Applications

FC IHC

Crossreactivity

Ca Hu Mk

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

CD1a is a ~49kDa single pass type 1 transmembrane glycoprotein containing a single Ig-like domain, expressed in association with beta 2 microglobulin. CD1a is expressed strongly by cortical thymocytes, and also by Langerhans cells and interdigitating cells. CD1a is involved in the presentation of lipids and glycolipids to NK cells.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1/Ag4.1 myeloma cells with spleen cells from Balb/c mice.

Positive Control for Immunohistochemistry:
Skin

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG2a|Clone No: 3H643 (NA1/34-HLK)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.|Purity: Purified by Ion Exchange chromatography|Immunogen: Human thymocytes|Specificity: Recognizes human CD1. Species Crossreactivity: canine and Cynomolgus monkey||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human thymocytes

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purity

Purified by Ion Exchange chromatography

Specificity

Recognizes human CD1. Species Crossreactivity: canine and Cynomolgus monkey

References

1. 1. McMichael, A. J. et al. (1979) A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur. J. Immunol. 9: 205-210, 2. Poulter, L. W. et al. (1986) Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies. Scand. J. Immunol. 24: 351-357, 3. Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leucocytes. Vet. Immunol. Immunopathol. 53: 329-334, 4. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross reactive antibodies. Exp. Anim. 49: 97-110.