CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (FITC)

Cat# C2255-01V-100ug

Size : 100ug

Brand : US Biological

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Phone : +1 850 650 7790


C2255-01V CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (FITC)

Clone Type
Monoclonal
Host
mouse
Source
bovine
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo
Shipping Temp
Blue Ice
Storage Temp
-20°C

C2255-01V precipitates a molecule of approximately 50kD and is considered to be the bovine homologue of Human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-83Y1 IgG2a Negative Control Murine (FITC)

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: CC8 |Host: mouse|Source: bovine|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with FITC.|Purity: Purified by Protein G affinity chromatography.|Specificity: Recognizes bovine CD4.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with FITC.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes bovine CD4.
References
1. Bensaid, A. et al. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4). Vet. Immunol. Immunopathol. 27: 51-54. 2. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets. Vet. Immunol. Immunopathol. 27: 215-231.