G-Protein, alpha, (Goa) (HRP)

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Go belongs to the pertussis toxin sensitive G-proteins. It consists of two subtypes of a-subunits (ao1 and a2) derived from alternative splicing of a single gene. ao1 differs from ao2 within the C-terminal third of the deduced amino acid sequence. Some of the literature suggests that Transducin is the generic name for the heterotrimeric G protein or possibly Gt. Transducin is composed of several subunits. G proteins are further categorized into different classes, including Go and Gi, based on their aa sequence. Goa is a subunit of Go. Goa could be one subgroup of Transducin. However, there is some literature stating that Go, Gi, and Transducin are all separate groups of G proteins.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:3000. Detects a single protein band of 39-42kD in bovine and rat membranes.
Optimal dilutions to be determined by researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.  

Note: Applications are based on unconjugated antibody.