Mouse Anti-Mouse H-2Db-BIOT
Cat# MBS673694-0,5mg
Size : 0.5mg
Brand : MyBiosource
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Phone : +1 850 650 7790
Mouse Anti-Mouse H-2db Secondary Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673694MBS673694 is a secondary antibody targeting Mouse Anti-Mouse H-2Db. Also known as Mouse Anti-Mouse H-2Db-BIOT. Reactive in Mouse. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
Mouse Anti-Mouse H-2Db, Secondary Antibody
Full Product Name
Mouse Anti-Mouse H-2Db-BIOT
Product Synonym Names
Mouse Anti-Mouse H-2Db - Biotin; Mouse Anti-Mouse H-2Db
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG2a kappa
Clone Number
27-11-13S
Host
Mouse
Species Reactivity
Mouse
Form/Format
Biotin (BIOT) Conjugate
Specificity
An epitope on the alpha chain of H-2Db
MAb 27-11-13 recognizes with the alpha 3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha 3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
MAb 27-11-13 recognizes with the alpha 3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha 3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Immunogen
C3H alpha BDF1 splenocytes1
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Applications Tested/Suitable for Mouse Anti-Mouse H-2Db secondary antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
Application Notes for Mouse Anti-Mouse H-2Db secondary antibody
Research Application(s): Complement-dependent cytoxicity assay
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Product References and Citations for Mouse Anti-Mouse H-2Db secondary antibody
1. Ozato, K., T.H. Hansen, and D.H. Sachs. 1980. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex. J. Immunol. 125:2473-2477.
2. Ozato, K., and D.H. Sachs. 1981. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126:317-321.
2. Ozato, K., and D.H. Sachs. 1981. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126:317-321.
Other Notes
Small volumes of Mouse Anti-Mouse H-2Db secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.