Mouse CD31/PECAM-1 Antibody : LE/AF
Cat# OASB00409
Size : 0.5mg
Brand : Aviva Systems Biology
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Phone : +1 850 650 7790
Mouse CD31/PECAM-1 Antibody : LE/AF (OASB00409)
| Datasheets/Manuals | Printable datasheet for Mouse CD31/PECAM-1 Antibody : LE/AF (OASB00409) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 390 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, ICC, IP, BL |
| Additional Information | Description: CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 interacts homotypically in cell adhesion assays. The monoclonal antibody 390 inhibits the aggregation of L cells transfected with a variant form of CD31. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | C3H/HeJ mouse hematopoietic progenitor cell line 32D |
| Concentration | 0.5 mg/mL |
| Specificity | CD31 |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform withcharacteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts ofwater. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidicconditions. Dilute azide- containing compounds in running water before discarding to avoid accumulation of potentiallyexplosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate R-phycoerythrin-Cyanine 7 conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 1 ug/106 cells <= 0.2 ug/106 cells <= 0.2 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 1, Immunoprecipitation 1, Immunohistochemistry (frozen sections) 4, Blocking of cell adhesion 1 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 1 Ug/106 cells BALB/c splenocytes were first incubated with purified rat anti-mouse CD31 and then with goat anti-rat Ig-FITC. Small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Storeat 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS Aliquot and store at or below - 20 C - The R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 C immediatelyupon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. |
| Reference | 1. Rosenblum, W.I., G.H. Nelson, B. Wormley, P. Werner, J. Wang, and C.C. Shih. 1996. Stroke 27:709 2. Buck, C.A., H.S. Baldwin, H. DeLisser, C. Mickanin, H.- M. Shen, G. Kennedy, A. Chen, J.M. Edelman, and S.M. Abelda. 1993. Comptes Rendus de l- Academie des Sciences Serie III- Sciences de la Vie- Life Sciences 316:838 3. DeLisser, H.M., P.J. newman, and S.M. Abelda. 1993. Curr. Topics Microbiol. Immunol. 184:37 4. Wyder, L., A. Vitaliti, H. Schneider, L.W. Hebbard, D.R. Moritz, M. Wittmer, M. Ajmo, and R. Klemenz. 2000. Cancer Res. 60:4682.. |
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| Gene Symbol | PECAM1 |
| Gene Full Name | Platelet endothelial cell adhesion molecule |
| Alias Symbols | Cd31, Pecam, C85791, PECAM-1 |
| NCBI Gene Id | 18613 |
| Protein Name | platelet endothelial cell adhesion molecule |
| Uniprot ID | Q08481 |
| Protein Accession # | NP_001027550.1 |
| Nucleotide Accession # | NM_001032378.2 |
