RayBio® G-Series Mouse Cytokine Antibody Array 2000. A combination of Mouse Cytokine Antibody Array G3, G4, & G5 Kit. Detects 144 Mouse Cytokines. Suitable for all liquid sample types.
High Density
Mouse Cytokine Array G2000
Mouse Cytokine Array G2000
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 144 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| 4-1BB (TNFRSF9/CD137)
| 6Ckine (CCL21)
| ACE
| ALK-1
| Amphiregulin
|
| Axl
| bFGF
| BLC (CXCL13)
| Cardiotrophin-1 (CT-1)
| CD27 (TNFRSF7)
|
| CD27 Ligand (TNFSF7)
| CD30 Ligand (TNFSF8)
| CD30 (TNFRSF8)
| CD36 (SR-B3)
| CD40 (TNFRSF5)
|
| CD40 Ligand (TNFSF5)
| Chordin
| CRG-2
| CTACK (CCL27)
| CTLA-4 (CD152)
|
| CXCL16
| Decorin
| DKK-1
| CD26 (DPPIV)
| Dtk
|
| E-Cadherin
| EGF
| Endoglin (CD105)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
| Epigen
| Epiregulin
| E-Selectin
| Fas Ligand (TNFSF6)
| Fc gamma RIIB (CD32b)
|
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| Galectin-1
| GCSF
| GITR (TNFRSF18)
|
| GITR Ligand (TNFSF18)
| GM-CSF
| Granzyme B
| Gas 1
| Gas 6
|
| HAI-1
| HGF
| HGFR
| ICAM-1 (CD54)
| IFN-gamma
|
| IGFBP-2
| IGFBP-3
| IGFBP-5
| IGFBP-6
| IGF-1
|
| IGF-2
| IL-1 beta (IL-1 F2)
| IL-1 R4 (ST2)
| IL-10
| IL-11
|
| IL-12 p40/p70
| IL-12 p70
| IL-13
| IL-15
| IL-17A
|
| IL-17B
| IL-17 RB
| IL-17E (IL-25)
| IL-17F
| IL-1 alpha (IL-1 F1)
|
| IL-1 RA (IL-1 F3)
| IL-2
| IL-2 R alpha
| IL-20
| IL-21
|
Species Detected
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array G2000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAM-CYT-G3-4.gal)
GAL (GAL-AAM-CYT-G3-8.gal)
GAL (GAL-AAM-CYT-G4-4.gal)
GAL (GAL-AAM-CYT-G4-8.gal)
GAL (GAL-AAM-CYT-G5-4.gal)
GAL (GAL-AAM-CYT-G5-8.gal)
Excel (AAM-CYT-G3_Analysis_Tool.xls)
Excel (AAM-CYT-G4_Analysis_Tool.xls)
Excel (AAM-CYT-G5_Analysis_Tool.xls)
citations
Obesity alters the lung myeloid cell landscape to enhance breast cancer metastasis through IL5 and GM-CSF
- Species:
- Mouse
- Sample Type:
- Serum (Obese and normal mouse models)
- Year:
- 2017
- Journal Name:
- Nature Cell Biology
- PubMed ID:
- 28737771
CXCR2 Inhibition Profoundly Suppresses Metastases and Augments Immunotherapy in Pancreatic Ductal Adenocarcinoma
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Pancreatic Cancer cells)
- Year:
- 2016
- Journal Name:
- Cancer Cell
- PubMed ID:
- 27265504
PTH Signaling in Osteoprogenitors Is Essential for B-Lymphocyte Differentiation and Mobilization
- Species:
- Mouse
- Sample Type:
- Bone Marrow Aspirate (Mone marrow supernates from control and Osx-PPR KO mice)
- Year:
- 2015
- Journal Name:
- Journal of Bone and Mineral Research
- PubMed ID:
- 26191777
Pro-Insulin-Like Growth Factor-II Ameliorates Age-Related Inefficient Regenerative Response by Orchestrating Self-Reinforcement Mechanism of Muscle Regeneration
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (tibialis anterior tissue lysate)
- Year:
- 2015
- Journal Name:
- Stem Cells
- PubMed ID:
- 25917344
Anti-inflammatory peptides from cardiac progenitors ameliorate dysfunction after myocardial infarction
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Cardiac progenitor cells, Skeletal myobalstas, and adipose-derived mesenchymal cells)
- Year:
- 2014
- Journal Name:
- Journal of the American Heart Association
- PubMed ID:
- 25468657