Mouse Cytokine Array GS4 (Glass Slide)

Cat# GSM-CYT-4-4

Size : 64SampleKit

Brand : RayBiotech

Contact local distributor :


Phone : +1 850 650 7790

Mouse Cytokine Array GS4

G-Series Mouse Cytokine Array 4 Kit. Semi-quantitative measurement of 40 mouse cytokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size16 Sample Kit, 32 Sample Kit, 64 Sample Kit
SpeciesMouse
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected40
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice
Storage-20°C

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
Amphiregulin
  • Gene Symbol: AREG
  • Accession #: P31955
  • ELISA Kit: RayBio Mouse AREG ELISA
Axl
  • Gene Symbol: Axl
  • Accession #: Q00993
  • ELISA Kit: RayBio Mouse Axl ELISA
CD27 Ligand
(TNFSF7)
  • Gene Symbol: CD70
  • Accession #: P32970
  • ELISA Kit: RayBio Mouse CD27 L ELISA
CD30
(TNFRSF8)
  • Gene Symbol: CD30
  • Accession #: Q60846
  • ELISA Kit: RayBio Mouse CD30 ELISA
CD40
(TNFRSF5)
  • Gene Symbol: CD40
  • Accession #: Q99NE2
  • ELISA Kit: RayBio Mouse CD40 ELISA
CXCL16
  • Gene Symbol: CXCL16
  • Accession #: Q5F2D5
  • ELISA Kit: RayBio Mouse CXCL16 ELISA
EGF
  • Gene Symbol: EGF
  • Accession #: E9QNX6
  • ELISA Kit: RayBio Mouse EGF ELISA
E-Selectin
  • Gene Symbol: SELE
  • Accession #: Q00690
  • ELISA Kit: RayBio Mouse E-Selectin ELISA
Fractalkine
(CX3CL1)
  • Gene Symbol: CX3CL1
  • Accession #: O35188
  • ELISA Kit: RayBio Mouse Fractalkine ELISA
GITR
(TNFRSF18)
  • Gene Symbol: TNFRSF18
  • Accession #: Q9JKR2
  • ELISA Kit: RayBio Mouse GITR ELISA
HGF
  • Gene Symbol: HGF
  • Accession #: Q53WS5
  • ELISA Kit: RayBio Mouse HGF ELISA
IGF-1
  • Gene Symbol: IGF1
  • Accession #: P05018
  • ELISA Kit: RayBio Mouse IGF-1 ELISA
IGFBP-2
  • Gene Symbol: IGFBP2
  • Accession #: P47877
  • ELISA Kit: RayBio Mouse IGFBP-2 ELISA
IGFBP-3
  • Gene Symbol: IGFBP3
  • Accession #: P47878
  • ELISA Kit: RayBio Mouse IGFBP-3 ELISA
IGFBP-5
  • Gene Symbol: IGFBP5
  • Accession #: Q07079
  • ELISA Kit: RayBio Mouse IGFBP-5 ELISA
IGFBP-6
  • Gene Symbol: IGFBP6
  • Accession #: Q91X24
  • ELISA Kit: RayBio Mouse IGFBP-6 ELISA
IL-1 ra
(IL-1 F3)
  • Gene Symbol: IL1RN
  • Accession #: P25085
  • ELISA Kit: RayBio Mouse IL-1 RA ELISA
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-17E
(IL-25)
  • Gene Symbol: IL25
  • Accession #: Q8VHH8
  • ELISA Kit: RayBio Mouse IL-17E ELISA
IL-17F
  • Gene Symbol: IL17F
  • Accession #: Q7TNI7
  • ELISA Kit: RayBio Mouse IL-17F ELISA
IL-2 R alpha
  • Gene Symbol: IL2RA
  • Accession #: Q61731
  • ELISA Kit: RayBio Mouse IL-2 R alpha ELISA
IL-20
  • Gene Symbol: IL20
  • Accession #: Q9JKV9
  • ELISA Kit: RayBio Mouse IL-20 ELISA
IL-23
  • Gene Symbol: IL23A; IL23B
  • Accession #: Q9EQ14; NM_016584
  • ELISA Kit: RayBio Mouse IL-23 ELISA
IL-28A
(IFN-lambda 2)
  • Gene Symbol: IFNL2
  • Accession #: Q4VK74
  • ELISA Kit: RayBio Mouse IL-28 ELISA
I-TAC
(CXCL11)
  • Gene Symbol: CXCL11
  • Accession #: Q9JHH5
  • ELISA Kit: RayBio Mouse I-TAC ELISA
MDC
(CCL22)
  • Gene Symbol: CCL22
  • Accession #: O88430
  • ELISA Kit: RayBio Mouse MDC ELISA
MIP-2
  • Gene Symbol: Cxcl2
  • Accession #: P10889
  • ELISA Kit: RayBio Mouse MIP-2 ELISA
MIP-3 alpha
(CCL20)
  • Gene Symbol: CCL20
  • Accession #: O89093
  • ELISA Kit: RayBio Mouse MIP-3 alpha ELISA
Osteopontin
(SPP1)
  • Gene Symbol: SPP1
  • Accession #: P19008
  • ELISA Kit: RayBio Mouse OPN ELISA
Osteoprotegerin
(TNFRSF11B)
  • Gene Symbol: TNFRSF11B
  • Accession #: O70202
  • ELISA Kit: RayBio Mouse OPG ELISA
Prolactin
  • Gene Symbol: PRL
  • Accession #: P06879
  • ELISA Kit: RayBio Mouse Prolactin ELISA
Pro-MMP-9
  • Gene Symbol: Mmp9
  • Accession #: Q80XI8
  • ELISA Kit: RayBio Mouse pro-MMP-9 ELISA
P-Selectin
  • Gene Symbol: SELP
  • Accession #: Q01102
  • ELISA Kit: RayBio Mouse P-Selectin ELISA
Resistin
  • Gene Symbol: RETN
  • Accession #: Q99P87
  • ELISA Kit: RayBio Mouse Resistin ELISA
SCF
  • Gene Symbol: KITLG
  • Accession #: P20826
  • ELISA Kit: RayBio Mouse SCF ELISA
SDF-1 alpha
(CXCL12 alpha)
  • Gene Symbol: CXCL12
  • Accession #: P40224
  • ELISA Kit: RayBio Mouse SDF-1 alpha ELISA
Thrombopoietin
(TPO)
  • Gene Symbol: THPO
  • Accession #: P40226
  • ELISA Kit: RayBio Mouse TPO ELISA
VCAM-1
(CD106)
  • Gene Symbol: VCAM1
  • Accession #: P29533
  • ELISA Kit: RayBio Mouse VCAM-1 ELISA
VEGF-A
  • Gene Symbol: VEGFA
  • Accession #: Q00731
  • ELISA Kit: RayBio Mouse VEGF-A ELISA
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAM-CYT-4.gal)
Excel (GSM-CYT-4_Analysis_Tool.xlsx)

citations

  • RAS/MEK/PI3K pathway inhibition augments response to CD40 agonism by targeting CD11b+ Bregs thereby overcoming melanoma PD1-resistance

    Species:
    Mouse
    Sample Type:
    Cell Culture Supernatant (culture supernatants of CD11b+, CD11b- B cells)
    Year:
    2026
    Journal Name:
    Nature Communications
    PubMed ID:
    41526341
  • FAM3A drives uncoupling of muscle lipid accumulation and insulin resistance depending on insulin receptor

    Year:
    2025
    Journal Name:
    Cell Death & Disease
    PubMed ID:
    41353211
  • Multi-omic analysis reveals Wharton's jelly mesenchymal stem cells shift the pro-inflammatory phenotype of fibroblast subpopulation to induce skin rejuvenation

    Species:
    Mouse
    Sample Type:
    Cell Culture Supernatant (skin tissues lysate supernatant)
    Year:
    2026
    Journal Name:
    Cell and Bioscience
    PubMed ID:
    42271436
  • Bacterial Lysate-Based Bifunctional mRNA Nanoformulation for Efficient Colon Cancer Immunogene Therapy

    Year:
    2024
    Journal Name:
    ACS Applied Materials & Interfaces
    PubMed ID:
    39397736
  • Inducing the “re-development state” of periodontal ligament cells via tuning macrophage mediated immune microenvironment

    Species:
    Mouse
    Sample Type:
    RAW264.7 cell
    Year:
    2023
    Journal Name:
    Journal of advanced research
  • Dynamic YAP expression in the non-parenchymal liver cell compartment controls heterologous cell communication

    Species:
    Mouse
    Sample Type:
    Serum (WT vs YAPKO mice)
    Year:
    2024
    Journal Name:
    Cellular and Molecular Life Sciences
    PubMed ID:
    38436764
  • Injury-induced interleukin-1 alpha promotes Lgr5 hair follicle stem cells de novo regeneration and proliferation via regulating regenerative microenvironment in mice

    Species:
    Mouse
    Sample Type:
    Dorsal skin tissues
    Year:
    2023
    Journal Name:
    Inflammation and regeneration
  • Aging-elevated inflammation promotes DNMT3A R878H-driven clonal hematopoiesis

    Species:
    Mouse
    Sample Type:
    Cell Culture Supernatant (bone marrow supernatant flushed femurs and tibias)
    Year:
    2022
    Journal Name:
    Acta Pharmaceutica Sinica B
    PubMed ID:
    35256939
  • Characteristics of Neutrophil Migration and Function in Acute Inflammation Induced by Zymosan and Carrageenan in the Mice Air Pouch Model

    Species:
    Mouse
    Sample Type:
    Other Body Fluid (air pouch lavage fluid exudate)
    Year:
    2024
    Journal Name:
    Inflammation
    PubMed ID:
    38902540
  • Correction: A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner

    Species:
    Human
    Sample Type:
    THP-1 cell, Human Dermal Lymphatic Endothelial Cells (HDLEC)
    Year:
    2022
    Journal Name:
    Angiogenesis