This mouse DBI (Diazepam Binding Inhibitor) ELISA kit is a sandwich immunoassay for the quantitative measurement of DBI, also known as acyl-CoA-binding protein, in mouse serum and plasma, supporting GABA-A receptor modulation and lipid metabolism research.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to DBI. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to DBI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DBI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DBI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DBI in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Effects of acyl-coenzyme A binding protein (ACBP)/diazepam-binding inhibitor (DBI) on body mass index; Cell Death Dis; 2021 Jun 9;12(6):599.; PMID: 34108446; A Joseph; Centre de Recherche des Cordeliers, Equipe labellisée par la Ligue contre le cancer, Inserm U1138, Université de Paris, Sorbonne Université, Paris, France.

Small volumes of DBI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2025156 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Diazepam Binding Inhibitor (DBI) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DBI. The ELISA analytical biochemical technique of the MBS2025156 kit is based on DBI antibody-DBI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DBI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DBI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).