Mouse H-2Db Antibody : LE/AF
Cat# OASB00723
Size : 0.5mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Mouse H-2Db Antibody : LE/AF (OASB00723)
| Datasheets/Manuals | Printable datasheet for Mouse H-2Db Antibody : LE/AF (OASB00723) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 27-11-13S |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, ICC, IP, CMCD |
| Additional Information | Description: The monoclonal antibody 27-11-13S recognizes with the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Dd, H-2Dq, H-2Ld and H-2Lq but not H-2Kb. Reactivity with haplotypes k, f, p, r, and s has not been observed. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | BDF1 mouse splenocytes |
| Concentration | 0.5 mg/mL |
| Specificity | H-2Db |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry1-3, Complement-dependent cytoxicity assay1,2 |
| Other Applications Data | Since applications vary, investigators are advised to determine the optimalworking dilution of the product that is appropriate for a specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Ozato, K., T.H. Hansen, and D.H. Sachs. 1980. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H- 2Ld antigen, theproducts of a third polymorphic locus of the mouse major histocompatibility complex. J. Immunol. 125:2473- 2477 2. Ozato, K., and D.H. Sachs. 1981. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H- 2b haplotype reveal genetic control of isotype expression. J. Immunol. 126:317- 321 3. Palmowski,M.J., M. Parker, K. Choudhuri, C. Chiu, M.F. Callan, P.A. van der Merwe, V. Cerundolo and K.G. Gould. 2009. J. Immunol 182:4565. |
|---|---|
| Gene Symbol | H2-D1 |
| Gene Full Name | H-2 class I histocompatibility antigen, D-B alpha chain |
| Alias Symbols | H-2D, H2-D, H2-K1 |
| NCBI Gene Id | 14964 |
| Protein Name | H-2 class I histocompatibility antigen, D-B alpha chain |
| Uniprot ID | P01899 |
| Protein Accession # | NP_034510.3 |
| Nucleotide Accession # | NM_010380.3 |
