General Info
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description: | This H3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
Information
| Gene Symbol: | H3c1.H3c8.H3c10.H3c11H |
| Gene ID: | 319152/319153/360198/9790815077/260423/3 |
| Uniprot ID: | H31_MOUSEH32_MOUSEH33_MOUSE< |
| Specificity: | This kit recognizes Mouse H3 in samples. No significant cross-reactivity or interference between Mouse H3 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Alternative ELISA Names | Histone H3.1 ELISA kitH3c1 ELISA kitH3a ELISA kitHist1h3a.H3c8 ELISA kitH3.1-221 ELISA kitH3g ELISA kitHist1h3g.H3c10 ELISA kitH3.1-291 ELISA kitH3h ELISA kitHist1h3h.H3c11 ELISA kitH3.1-I ELISA kitH3i ELISA kitHist1h3i ELISA kitHistone H3.2 ELISA kitH3-Clustered Histone 13 ELISA kitH3-Clustered Histone 14 ELISA kitH3-Clustered Histone 15 ELISA kitH3-Clustered Histone 2 ELISA kitH3-Clustered Histone 3 ELISA kitH3-Clustered Histone 4 ELISA kitH3-Clustered Histone 6 ELISA kitH3-Clustered Histone 7 ELISA kitH3c2 ELISA kitH3-53 ELISA kitH3.2 ELISA kitH3b ELISA kitHist1h3b.H3c3 ELISA kitH3-143 ELISA kitHist1h3c.H3c4 ELISA kitH3-B ELISA kitHist1h3d.H3c6 ELISA kitH3-F ELISA kitHist1h3e.H3c7 ELISA kitH3.2-221 ELISA kitH3f ELISA kitHist1h3f.H3c13 ELISA kitH3.2-616 ELISA kitHist2h3b.H3c14 ELISA kitH3.2-615 ELISA kitHist2h3c1 ELISA kitHist2h3ca1.H3c15 ELISA kitH3.2-614 ELISA kitHist2h3c2 ELISA kitHist2h3ca2 ELISA kitHistone H3.3 ELISA kitH3-3a ELISA kitH3.3a ELISA kitH3f3a.H3-3b ELISA kitH3.3b ELISA kitH3f3b ELISA kitHistone H3.1 ELISA kitH3c1 ELISA kitH3a ELISA kitHist1h3a.H3c8 ELISA kitH3.1-221 ELISA kitH3g ELISA kitHist1h3g.H3c10 ELISA kitH3.1-291 ELISA kitH3h ELISA kitHist1h3h.H3c11 ELISA kitH3.1-I ELISA kitH3i ELISA kitHist1h3i ELISA kitHistone H3.2 ELISA kitH3-Clustered Histone 13 ELISA kitH3-Clustered Histone 14 ELISA kitH3-Clustered Histone 15 ELISA kitH3-Clustered Histone 2 ELISA kitH3-Clustered Histone 3 ELISA kitH3-Clustered Histone 4 ELISA kitH3-Clustered Histone 6 ELISA kitH3-Clustered Histone 7 ELISA kitH3c2 ELISA kitH3-53 ELISA kitH3.2 ELISA kitH3b ELISA kitHist1h3b.H3c3 ELISA kitH3-143 ELISA kitHist1h3c.H3c4 ELISA kitH3-B ELISA kitHist1h3d.H3c6 ELISA kitH3-F ELISA kitHist1h3e.H3c7 ELISA kitH3.2-221 ELISA kitH3f ELISA kitHist1h3f.H3c13 ELISA kitH3.2-616 ELISA kitHist2h3b.H3c14 ELISA kitH3.2-615 ELISA kitHist2h3c1 ELISA kitHist2h3ca1.H3c15 ELISA kitH3.2-614 ELISA kitHist2h3c2 ELISA kitHist2h3ca2 ELISA kitHistone H3.3 ELISA kitH3-3a ELISA kitH3.3a ELISA kitH3f3a.H3-3b ELISA kitH3.3b ELISA kitH3f3b ELISA kit |
| Specificity | This kit recognizes Mouse H3 in samples. No significant cross-reactivity or interference between Mouse H3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-106 | 99 |
| EDTA plasma (n=8) | 91-101 | 96 |
| Cell culture media (n=8) | 90-103 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.51 | 1.24 | 4.86 | 0.53 | 1.35 | 5.32 |
| Standard deviation | 0.03 | 0.07 | 0.16 | 0.04 | 0.07 | 0.28 |
| CV (%) | 5.88 | 5.65 | 3.29 | 7.55 | 5.19 | 5.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance