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> Mouse IgM Isotype Control FITC Antibody

Mouse IgM Isotype Control FITC Antibody

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Cat# orb154495-0.1mg

Size : 0.1mg

Brand : Biorbyt

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Phone : +1 850 650 7790

Mouse IgM Isotype Control FITC Antibody

SKU: orb154495
This product can be custom made

Description

Mouse monoclonal antibody conjugated to FITC which recognizes IgM that is the major component of the natural antibodies and is also the first class of antibodies produced during a primary antibody response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen.

Research Area

Stem Cells

Images & Validation

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Tested ApplicationsFC
Application Notes
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Key Properties

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Antibody TypeSecondary Antibody
ClonalityMonoclonal
IsotypeMouse IgM
Clone No.PFR-03
PurificationPurified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationFITC

Storage & Handling

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StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesStabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Quick Database Links

NCBI Gene ID
35

NCBI Gene Details

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Failed to load NCBI Gene data

Protocol Information

FC
Flow Cytometry
View Protocol