Mouse IL-4 Antibody : LE/AF
Cat# OASB01245
Size : 0.5mg
Brand : Aviva Systems Biology
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Phone : +1 850 650 7790
Mouse IL-4 Antibody : LE/AF (OASB01245)
| Datasheets/Manuals | Printable datasheet for Mouse IL-4 Antibody : LE/AF (OASB01245) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | BVD4-1D11 |
| Isotype | IgG2b |
| Host | Rat |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | ELISPOT, FC, IHC-F, IHC-P, ICC, WB, IP, MP |
| Reconstitution and Storage | Store at -20C |
| Immunogen | E. coli-expressed mouse IL-4 |
| Concentration | 0.5 mg/mL |
| Specificity | IL-4 |
| Characterization | To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform tocharacteristics of a standard reference reagent. |
| Application Info | ELISA Capture: BVD4-1D11 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels. 1-4 Purified BVD4-1D11 antibody should be paired with biotinylated BVD6-24G2 antibody (OASB01247) as the detection antibody, with purified recombinant mouse IL-4 as the standard. Immunofluorescence/Flow Cytometry: BVD4-1D11 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b (OASB00115) should be used as a specificity control. Western Blotting: The purified BVD4-1D11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig, OASB01897) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-4 under reducing conditions. Neutralization: The LE/AF form of BVD4-1D11 can be used for neutralizing mouse IL-4 bioactivity. A suitable LE/AF Rat IgG2b (OASB00124) should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Reagents are stable for the period shown on the label if stored as directed. |
| Reference | 1. Abrams, J.S., M.- G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti- cytokine monoclonalantibody development: Immunoassay of IL- 10 and IL- 5 in clinical samples. Immunol. Rev. 127:5- 24 2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells inmurine peripheral blood and sera. J. Immunol. Meth. 166:201- 214 3. Finkelman, F., K. Madden, S. Morris, J. Holmes, N. Bolani,, I. Jkatona, and C. Maliszewski. 1993. Anti- cytokine antibodies as carrierproteins. J. Immunol. 151:1235- 1244 4. Litton, M., B. Sander, E. Murphy, A. O'Garra, and J. Abrams. 1994. Early expression of cytokines in lymph nodes after treatment in vivowith Staphylococcus enterotoxin B. J. Immunol. Meth. 175:47- 58. |
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| Gene Symbol | IL4 |
| Gene Full Name | Interleukin-4 |
| Alias Symbols | Il, Il-4, BSF-1 |
| NCBI Gene Id | 16189 |
| Protein Name | interleukin-4 |
| Uniprot ID | P07750 |
| Protein Accession # | NP_067258.1 |
| Nucleotide Accession # | NM_021283.2 |
