The RayBio® mouse inflammation cytokine array kit is a membrane-based multiplex assay to simultaneously detect 40 mouse inflammatory factors. Suitable for all liquid sample types.
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Mouse Inflammation Array C1
Mouse Inflammation Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 40 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Fas Ligand (TNFSF6)
|
Fractalkine (CX3CL1)
| GCSF
| GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1)
|
IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
| IL-6
|
IL-9
| IL-10
| IL-12 p40/p70
| IL-12 p70
| IL-13
|
IL-17A
| I-TAC (CXCL11)
| KC (CXCL1)
| Leptin
| LIX
|
Lymphotactin (XCL1)
| MCP-1 (CCL2)
| M-CSF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
MIP-1 gamma
| RANTES (CCL5)
| SDF-1 alpha (CXCL12 alpha)
| I-309 (TCA-3/CCL1)
| TECK (CCL25)
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
|
Typical Data
Kit Components
- Mouse Inflammation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
CD8+ T cell depletion prevents neuropathology in a mouse model of globoid cell leukodystrophy
- Species:
- Mouse
- Sample Type:
- brain tissue
- Year:
- 2023
- Journal Name:
- Journal of Experimental Medicine
Reversal of neurological deficits by painless nerve growth factor in a mouse model of Rett syndrome
- Species:
- Mouse
- Sample Type:
- brain
- Year:
- 2023
- Journal Name:
- Brain
Controlled Release of Cold Atmospheric Plasma by Gelatin Scaffold Enhances Wound Healing via Macrophage Modulation
- Year:
- 2025
- Journal Name:
- ACS Appl Mater Interfaces
- PubMed ID:
- 40013441
Loss of microRNA-15a/16-1 function promotes neuropathological and functional recovery in experimental traumatic brain injury
- Year:
- 2024
- Journal Name:
- JCI insight
- PubMed ID:
- 38912585
SAP deletion promotes malignant insulinoma progression by inducing CXCL12 secretion from CAFs via the CXCR4/p38/ERK signalling pathway
- Year:
- 2024
- Journal Name:
- Journal of Cellular and Molecular Medicine
- PubMed ID:
- 38766687
Targeted ablation of signal transducer and activator of transduction 1 alleviates inflammation by microglia/macrophages and promotes long-term recovery after ischemic stroke
- Species:
- Mouse
- Sample Type:
- brain tissue
- Year:
- 2023
- Journal Name:
- Journal of Neuroinflammation
A Novel Mix of Polyphenols and Micronutrients Reduces Adipogenesis and Promotes White Adipose Tissue Browning via UCP1 Expression and AMPK Activation
- Species:
- Mouse
- Sample Type:
- 3T3-L1 cell
- Year:
- 2023
- Journal Name:
- Cells
Decreased bone resorption in Ezh2 myeloid cell conditional knockout mouse model
- Species:
- Mouse
- Sample Type:
- spine
- Year:
- 2023
- Journal Name:
- FASEB JOURNAL
Soybean (Glycine max) INFOGEST Colonic Digests Attenuated Inflammatory Responses Based on Protein Profiles of Different Varieties
- Species:
- Mouse
- Sample Type:
- RAW 264.7 cell
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
Distinct Plasma Immune Profile in ALS Implicates sTNFR-II in pAMPK/Leptin Homeostasis
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences