RayBio® Mouse Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Mouse Leptin ELISA Kit
Mouse Leptin ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 4 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 4 pg/ml - 1000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF- 1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 10 - 40 fold |
| Accession Number | P41160 |
| Gene Id | 16846 |
| Gene Symbols | LEP, OB |
| Protein Name / Synonyms | Leptin (Obesity factor) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
citations
OLFR734 Mediates Glucose Metabolism as a Receptor of Asprosin
- Species:
- Mouse
- Sample Type:
- Plasma (Asprosin in glucose metabolism mouse model)
- Year:
- 2019
- Journal Name:
- Cell Metabolism
- PubMed ID:
- 31230984
Sema7A protects against high-fat diet-induced obesity and hepatic steatosis by regulating adipo/lipogenesis
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Molecular metabolism
Leptin/obR signaling exacerbates obesity-related neutrophilic airway inflammation through inflammatory M1 macrophages
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Molecular Medicine
Transient Receptor Potential (TRP) based polypharmacological combination stimulates energy expending phenotype to reverse HFD-induced obesity in mice
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- LIFE SCIENCES
The Effect of Orally Administered Δ9-Tetrahydrocannabinol (THC) and Cannabidiol (CBD) on Obesity Parameters in Mice
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
A novel combination of sitagliptin and melatonin ameliorates T2D manifestations: studies on experimental diabetic models
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Journal of Endocrinological Investigation
β-Caryophyllene: A Therapeutic Alternative for Intestinal Barrier Dysfunction Caused by Obesity
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Molecules
Methanolic fruit extract of Myrica nagi protects the hypothalamus and attenuates inflammation associated with gold thioglucose- and high-fat diet-induced obesity via various adipokines
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Journal of Ayurveda and integrative medicine
Intervention of probiotic L. reuteri fermented milk as an adjuvant to combat protein energy malnourishment induced gut disturbances in albino mice.
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2017
- Journal Name:
- Journal of Functional Foods
Orexin A regulates plasma insulin and leptin levels in a time-dependent manner following a glucose load in mice
- Species:
- Mouse
- Sample Type:
- Plasma (Glucose Tolerance testing of high fat diet C57BL/6 mice)
- Year:
- 2015
- Journal Name:
- Diabetologia
- PubMed ID:
- 25813215
