Mouse Ly-6G/GR-1 Antibody : CY5
Cat# OASB00690
Size : 0.1mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Mouse Ly-6G/GR-1 Antibody : CY5 (OASB00690)
| Datasheets/Manuals | Printable datasheet for Mouse Ly-6G/GR-1 Antibody : CY5 (OASB00690) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | RB6-8C5 |
| Isotype | IgG2b |
| Host | Rat |
| Conjugation | Cyanine 5 |
| Application | FC, IHC-F |
| Additional Information | Description: Ly-6G/Ly-6C, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. In the periphery, Ly-6G/Ly-6C is expressed predominantly on neutrophils. |
| Reconstitution and Storage | Store at 2-8C |
| Concentration | 0.1 mg/mL |
| Specificity | Ly-6G/Ly-6C |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative flow data are included in this productinsert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate Cyanine 5 conjugate R-phycoerythrin-Cyanine 5.5 (R-PE-CY5.5) conjugate R-phycoerythrin-Cyanine 7 (R-PE-CY7) conjugate Allophycocyanin -Cyanine 7 (APC-CY7)conjugate <=1 ug/106 cells <=1 ug/106 cells <=0.2 ug/106 cells <=0.05 ug/106 cells <=0.2 ug/106 cells <=0.2 ug/106 cells <=0.2 ug/106 cells <=0.2 ug/106 cells <=0.2 ug/106 cells |
| Application Info | Flow cytometry 1-4, Immunohistochemistry (frozen sections) 5, Immunoprecipitation 1, In vitro complement-dependent cytotoxicity assays 7, Western blotting 6, In vivo depletion of Ly-6G+ cells 8-12 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 1 Ug/106 cells BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat antimouse Ly-6G-FITC. Large cells were then gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg ofpurified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives oramine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg of 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5), R- phycoerythrin- Cyanine 7(R- PE- CY 7) and Allophycocyanin - Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Fleming, T.J., M.L. Fleming and T. R. Malek. 1993. J. Immunol. 151:2399 2. Hestdal, K., F.W. Ruscetti, J.N. Ihle, et al. 1991. J. Immunol. 147:22 3. Lewinsohn, D.M., R.F. Bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313 4. Lagasse, E., and I.L. Weissman. 1996. J. Immunol. Meth. 197:139 5. Ohki,Y., B. Heissig, Y. Sato, H. Akiyama, Z. Zhu, D.J. Hicklin, K. Shimada, H. Ogawa, H. Daida, K. Hattori, and A. Ohsaka. FASEB J. . . . .. . ….. 2005;19:2005- 2007 6. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, et al. 1988. Eur. J. Immunol. 18:1819 7. Brummer, E., A.M. Sugar, and D.A. Stevens. 1984. J. Leukocyte Biol. 36:505 8. Stoppacciaro, A., C. Melani, M. Parenza, et al. 1993. J. Exp. Med. 178:151 9. Tumpey, T.M., S.- H. Chen, J.E. Oakes, and R.N. Lausch. 1996. J. Virol. 70:898 10. Conlan, J.W., and.J. North. 1994. J. Exp. Med. 179:259 11. Czuprynski, C.J., J.F. Brown, N. Maroushek, R.D. Wagner, and H. Steinberg. 1994. J. Immunol. 152:1836 12. Rakhmilevich, A.L. 1995. J. Leukoc. Biol. 57:827. |
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| Gene Symbol | LY6G, LY6C1 |
| Gene Full Name | Lymphocyte antigen 6G |
| Alias Symbols | Gr, Gr-, Gr1, Gr-1, Ly-6, Ly-6G |
| NCBI Gene Id | 546644; 17067 |
| Protein Name | lymphocyte antigen 6G |
| Uniprot ID | P35461, P0CW02 |
