Mouse monoclonal antibody AF700 labled to CD33

Cat# CFO8518-100

Size : 100Assays

Brand : Cohesion Biosciences

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Phone : +1 850 650 7790

Product NameAnti-CD33 Antibody-AF700 labled
Cat No: CFO8518
Source: Mouse
Reactivity: H
Applications: IF, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Description: Mouse monoclonal antibody AF700 labled to CD33
Immunogen: Native purified human CD33.
Purification: The antibody was purified by affinity chromatography.
Clonality: Monoclonal (clone: WM53)
Conjugation: AF700
Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Dilution: 10 μl / assay
Gene Symbol: CD33
Alternative Names: SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33
Entrez Gene (Human):
SwissProt (Human):
Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.