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Mouse OPG ELISA Kit
Mouse OPG ELISA Kit
RayBio® Mouse Osteoprotegerin (TNFRSF11B) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 1 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 1200 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 20 - 200 fold |
| Accession Number | O08712 |
| Gene Id | 18383 |
| Gene Symbols | TNFRSF11B, OCIF, OPG |
| Protein Name / Synonyms | Tumor necrosis factor receptor superfamily member 11B (Osteoclastogenesis inhibitory factor) (Osteoprotegerin) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Mouse OPG qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse OPG ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Osteoprotegerin (TNFRSF11B / OPG), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Cannabinoid Receptor 2 Agonist JWH-015 Inhibits Interleukin-1β-Induced Inflammation in Rheumatoid Arthritis Synovial Fibroblasts and in Adjuvant Induced Arthritis Rat via Glucocorticoid Receptor
- Species:
- Rat
- Sample Type:
- Serum (AIA rats, JWH-015 treated)
- Year:
- 2019
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 31139184
Biomechanical dysregulation of SGK-1 dependent aortic pathologic markers in hypertension
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2024
- Journal Name:
- Frontiers in Cardiovascular Medicine
- PubMed ID:
- 38903966
Postnatal loss of the insulin receptor in osteoprogenitor cells does not impart a metabolic phenotype
- Species:
- Mouse
- Sample Type:
- Serum (OIRKO and control mice)
- Year:
- 2020
- Journal Name:
- Scientific Reports
- PubMed ID:
- 32483283
Endogenous neutralization of TGF-β and IL-6 ameliorates septic arthritis by altering RANKL/OPG interaction in lymphocytes
- Species:
- Mouse
- Sample Type:
- lymphocytes
- Year:
- 2022
- Journal Name:
- Molecular Immunology
Dual function of ERR in breast cancer and bone metastasis formation: implication of VEGF and osteoprotegerin.
- Species:
- Mouse
- Sample Type:
- Conditioned Media (BO2-CT(1/2), BO2- ERRa-WT-1, and BO2-FRT-ERRaDAF2)
- Year:
- 2011
- PubMed ID:
- 21734015
Simultaneous neutralization of TGF-β and IL-6 attenuates Staphylococcus aureus-induced arthritic inflammation through differential modulation of splenic and synovial macrophages
- Species:
- Mouse
- Sample Type:
- synovial macrophages
- Year:
- 2023
- Journal Name:
- Scandinavian Journal of Immunology
Systemic Osteoprotegerin Gene Therapy Restores Tumor-induced Bone Loss in a Therapeutic Model of Breast Cancer Bone Metastasis
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2008
- Journal Name:
- Molecular Therapy
Heterotopic bone formation derived from multipotent stromal cells is not inhibited in aged mice
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Bone marrow-derived MSCs and osteoblast-like bone stromal cells)
- Year:
- 2014
- Journal Name:
- Cytotherapy
Genistein affects osteoblastic MC3T3-E1 cells both through estrogen receptor and BMP-Smad signaling pathways
- Species:
- Mouse
- Sample Type:
- Conditioned Media (MC3T3-E1 cells)
- Year:
- 2013
- Journal Name:
- Kawasaki Medical Journal
Rapamycin increases neuroblastoma xenograft and host stromal derived osteoprotegerin inhibiting osteolytic bone disease in a bone metastasis model
- Species:
- Mouse
- Sample Type:
- Serum (xenografted human tumors into CB-17 SCID mice)
- Year:
- 2013
- Journal Name:
- Journal of Pediatric Surgery
- PubMed ID:
- 23331792