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Mouse SDF-1 alpha ELISA Kit
Mouse SDF-1 alpha ELISA Kit
RayBio® Mouse SDF-1 alpha (CXCL12 alpha) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 3 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 3 pg/ml - 600 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P40224 |
| Gene Id | 20315 |
| Gene Symbols | CXCL12, SDF1 |
| Protein Name / Synonyms | Stromal cell-derived factor 1 (SDF-1) (12-O-tetradecanoylphorbol 13-acetate repressed protein 1) (TPAR1) (C-X-C motif chemokine 12) (Pre-B cell growth-stimulating factor) (PBSF) (Thymic lymphoma cell-stimulating factor) (TLSF) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Mouse SDF-1 alpha qIPCR ELISA Kit
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RayPlex® Mouse SDF-1 alpha Bead Assay
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse SDF-1 alpha ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified SDF-1 alpha (CXCL12 alpha), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
LNP-RNA-engineered adipose stem cells for accelerated diabetic wound healing
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (conditioned medium of ASCs)
- Year:
- 2024
- Journal Name:
- Nature Communications
- PubMed ID:
- 38272900
Cxcl9high macrophages recruit circulating Cxcr3+ plasmablasts into kidneys to promote pathogenesis of lupus nephritis mice
- Species:
- Mouse
- Sample Type:
- Tissue Lysate / Homogenate (kidney lysates)
- Year:
- 2025
- Journal Name:
- Communications Biology
- PubMed ID:
- 41068254
Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageing
- Species:
- Mouse
- Sample Type:
- bone marrow (BM)
- Year:
- 2023
- Journal Name:
- Nature Cell Biology
Remodeling of the osteoimmune microenvironment after biomaterials implantation in murine tibia: Single-cell transcriptome analysis
- Species:
- Mouse
- Sample Type:
- neutrophils
- Year:
- 2022
- Journal Name:
- Bioactive materials
A Multimodal Scaffold for SDF1 Delivery Improves Cardiac Function in a Rat Subacute Myocardial Infarct Model
- Year:
- 2023
- Journal Name:
- Acs Applied Materials & Interfaces
Inhibition of SRC-mediated integrin signaling in bone marrow niche enhances hematopoietic stem cell function
- Year:
- 2022
- Journal Name:
- iScience
- PubMed ID:
- 36204266
Repairing sciatic nerve injury with an EPO-loaded nerve conduit and sandwiched-in strategy of transplanting mesenchymal stem cells.
- Species:
- Rat
- Sample Type:
- Conditioned Media (adipose-derived Mesenchymal Stem cells)
- Year:
- 2017
- Journal Name:
- Biomaterials
- PubMed ID:
- 28732247
Valproic acid restores the down-regulation of SDF-1 following kidney ischemia; experimental validation of a mathematical prediction
- Species:
- Mouse
- Sample Type:
- Tissue Lysate / Homogenate (kidney tissue lysates)
- Year:
- 2020
- Journal Name:
- Research in Pharmaceutical Sciences
- PubMed ID:
- 32582359
Myelopoiesis is regulated by osteocytes through Gs-dependent signaling
- Species:
- Mouse
- Sample Type:
- Conditioned Media (osteocyte-enriched bone explants)
- Year:
- 2013
- Journal Name:
- Blood
- PubMed ID:
- 23160461
SDF-1α/OPF/BP Composites Enhance the Migrating and Osteogenic Abilities of Mesenchymal Stem Cells
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (culture medium from SDF-1α, OPF, BP composites)
- Year:
- 2021
- Journal Name:
- Stem Cells International
- PubMed ID:
- 34434236
