General Information
| Short Description | This TIMP-1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | Capture immunogen: Recombinant Mouse TIMP-1 protein.Detection Immunogen: Recombinant Mouse TIMP-1 protein. |
Additional Info
| Post Translational Modifications | The activity of TIMP1 is dependent on the presence of disulfide bonds. N-glycosylated. |
| Function | Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. |
| Protein Name | Metalloproteinase Inhibitor 1Collagenase Inhibitor 16c8 FibroblastErythroid-Potentiating ActivityEpaTpa-S1Tpa-Induced ProteinTissue Inhibitor Of Metalloproteinases 1Timp-1 |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Metalloproteinase Inhibitor 1 antibody pairCollagenase Inhibitor 16c8 Fibroblast antibody pairErythroid-Potentiating Activity antibody pairEpa antibody pairTpa-S1 antibody pairTpa-Induced Protein antibody pairTissue Inhibitor Of Metalloproteinases 1 antibody pairTimp-1 antibody pairTimp1 antibody pairTimp antibody pairTimp-1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse TIMP-1 (Metalloproteinase Inhibitor 1) Antibody Pair (STJAP00267)
This antibody pair detects mouse TIMP-1 (Metalloproteinase Inhibitor 1), corresponding to UniProt entry P12032. Reactivity has been established for mouse samples only.
The capture antibody is an unconjugated rat monoclonal antibody and the detection antibody is a biotin-conjugated goat polyclonal antibody. Both components are supplied at 0.5 mg/mL, formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
The capture antibody was purified by Protein A or G, while the detection antibody was purified by antigen affinity chromatography. Both antibodies were raised against recombinant mouse TIMP-1 protein as the immunogen.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.