Mouse Viperin ELISA Kit

Cat# MBS456929-48

Size : 48Tests

Brand : MyBiosource

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Viperin (RSAD2) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS456929

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Viperin in mouse serum, plasma, tissue homogenates or other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been… More details

Product Overview

Online Datasheet in PDF
Product Name
Viperin (Rsad2), ELISA Kit
Full Product Name
Mouse Viperin ELISA Kit
Product Gene Name
Rsad2 elisa kit
Product Synonym Names
Cig5; RSAD2; vig1; Radical S-Adenosyl Methionine Domain Containing 2; Viperin,Endoplasmic Reticulum-Associated,Interferon-Inducible; Cytomegalovirus-induced gene 5
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates or other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
<0.072ng/mL.
Specificity
This assay has high sensitivity and excellent specificity for detection of Viperin. No significant cross-reactivity or interference between Viperin and analogues was observed.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Viperin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Viperin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
41,524 Da
3D Structure
ModBase 3D Structure for Q8CBB9
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of Rsad2 elisa kit

Mouse RSAD2 ELISA Kit – Cat# MBS456929

Other Notes

Small volumes of Rsad2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rsad2 purchase

MBS456929 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Viperin (Rsad2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Rsad2. The ELISA analytical biochemical technique of the MBS456929 kit is based on Rsad2 antibody-Rsad2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Rsad2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Rsad2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Rsad2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
radical S-adenosyl methionine domain-containing protein 2
NCBI Official Synonym Full Names
radical S-adenosyl methionine domain containing 2
NCBI Official Symbol
Rsad2
NCBI Official Synonym Symbols
Vig1; cig5; 2510004L01Rik
UniProt Protein Name
Radical S-adenosyl methionine domain-containing protein 2
UniProt Synonym Protein Names
Viperin; Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible
Protein Family
Radical S-adenosyl methionine domain-containing protein
UniProt Gene Name
Rsad2
NCBI Protein Information
radical S-adenosyl methionine domain-containing protein 2

UniProt Comments for Rsad2

RSAD2: Interferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. Belongs to the radical SAM superfamily. RSAD2 family.

Chromosomal Location of Human Ortholog: 12|12 A2

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; fibrillar center; lipid droplet; membrane; mitochondrial inner membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: 4 iron, 4 sulfur cluster binding; catalytic activity; iron-sulfur cluster binding; metal ion binding; protein binding; protein self-association

Biological Process: CD4-positive, alpha beta T cell differentiation; defense response to virus; immune system process; innate immune response; negative regulation of protein secretion; negative regulation of viral genome replication; ossification; positive regulation of toll-like receptor 7 signaling pathway; positive regulation of toll-like receptor 9 signaling pathway; regulation of ossification; response to virus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Rsad2 elisa kit

 Reference Product  PubMed Publications
 Ifit1 elisa kit  >18 publications with Rsad2 and Ifit1
 Mx2 elisa kit  >11 publications with Rsad2 and Mx2
 Usp18 elisa kit  >10 publications with Rsad2 and Usp18
 Ifit3 elisa kit  >9 publications with Rsad2 and Ifit3
 Irf7 elisa kit  >9 publications with Rsad2 and Irf7
 Ifit2 elisa kit  >8 publications with Rsad2 and Ifit2
 Ifih1 elisa kit  >6 publications with Rsad2 and Ifih1
 Dhx58 elisa kit  >3 publications with Rsad2 and Dhx58
 Cmpk2 elisa kit  >2 publications with Rsad2 and Cmpk2
 Oasl1 elisa kit  >1 publications with Rsad2 and Oasl1

Pathways associated with Rsad2 elisa kit

 Products by Pathway  Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram

Diseases associated with Rsad2 elisa kit

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >16 publications with Rsad2 and Liver Diseases
 Inflammation Antibodies  >15 publications with Rsad2 and Inflammation
 Nervous System Diseases Antibodies  >13 publications with Rsad2 and Nervous System Diseases
 Influenza, Human Antibodies  >7 publications with Rsad2 and Influenza, Human
 Cardiovascular Diseases Antibodies  >5 publications with Rsad2 and Cardiovascular Diseases
 Necrosis Antibodies  >4 publications with Rsad2 and Necrosis
 Disease Models, Animal Antibodies  >4 publications with Rsad2 and Disease Models, Animal
 Carcinoma, Hepatocellular Antibodies  >3 publications with Rsad2 and Carcinoma, Hepatocellular
 Kidney Diseases Antibodies  >3 publications with Rsad2 and Kidney Diseases
 Lung Diseases Antibodies  >3 publications with Rsad2 and Lung Diseases

Organs/Tissues associated with Rsad2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >31 publications with Rsad2 and Blood
 Liver Antibodies  >14 publications with Rsad2 and Liver
 Joint Antibodies  >6 publications with Rsad2 and Joint
 Kidney Antibodies  >6 publications with Rsad2 and Kidney
 Bone Antibodies  >5 publications with Rsad2 and Bone
 Embryonic Tissue Antibodies  >3 publications with Rsad2 and Embryonic Tissue
 Spleen Antibodies  >3 publications with Rsad2 and Spleen
 Bone Marrow Antibodies  >3 publications with Rsad2 and Bone Marrow
 Eye Antibodies  >2 publications with Rsad2 and Eye
 Thymus Antibodies  >1 publications with Rsad2 and Thymus