Mycoplasma pneumoniae (MP) Antibody (IgG) BioAssay™ ELISA Kit (Human)

Sample Type:
Serum

Intended Use:
For the qualitative determination of human mycoplasma pneumoniae (MP) antibody (IgG) concentrations in serum.

Sensitivity:
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Range:
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Specificity:
This assay has high sensitivity and excellent specificity for detection of human mycoplasma pneumoniae (MP) antibody (IgG). No significant cross-reactivity or interference between human mycoplasma pneumoniae (MP) antibody (IgG) and analogues was observed.

Test Principle:
This BioAssay™ employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human mycoplasma pneumoniae (MP) antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Kit Components:
Coated assay plate, 1x96 wells
Negative Control, 1x0.8ml
Positive Control, 1x0.8ml
Sample Diluent, 1x20ml
HRP-conjugate, 1x10ml
Wash Buffer (25X), 1x20ml
Substrate A, 1x5ml
Substrate B, 1x5ml
Stop Solution, 1x5ml
Adhesive Strip (For 96 wells), 4x strips
Instruction manual

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.