Neo DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate
Cat# NB-58-0021
Size : 500ml
Brand : Neo Biotech
Description
Neo DMEM High Glucose (4.5 g/L), with L-Glutamine and Sodium Pyruvate is a liquid basal medium supplying nutrients for mammalian cell culture. Its increased glucose content supports cells with higher energy demands.
Applications include primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, and cell lines such as HeLa, HEK 293, Cos-7, and PC-12.

Product Features
- High glucose: 4.5 g/L.
- Contains L-glutamine, sodium pyruvate, and phenol red.
- Bicarbonate-buffered formulation.
- Sterile-filtered; 500 mL format.
Product Specifications
| Property | Details |
|---|---|
| Appearance | Clear red-orange solution |
| Volume | 500 mL |
| L-Glutamine | 584 mg/L |
| Sodium Pyruvate | 110 mg/L |
| Optimal CO? Concentration | 8.5% |
| Intended Use | Research and further manufacturing only |
Storage and Shipping
| Property | Details |
|---|---|
| Storage | 2–8°C, protected from light |
| After Opening | 4°C; use within 6–8 weeks |
| Shipping | Ambient temperature |
Formulation — Amino Acids
| Component | Concentration (mg/L) |
|---|---|
| Glycine | 30.00 |
| L-Arginine HCl | 84.00 |
| L-Cystine 2HCl | 62.57 |
| L-Glutamine | 584.00 |
| L-Histidine HCl·H?O | 42.00 |
| L-Isoleucine | 105.00 |
| L-Leucine | 105.00 |
| L-Lysine HCl | 146.00 |
| L-Methionine | 30.00 |
| L-Phenylalanine | 66.00 |
| L-Serine | 42.00 |
| L-Threonine | 95.00 |
| L-Tryptophan | 16.00 |
| L-Tyrosine disodium salt dihydrate | 103.79 |
| L-Valine | 94.00 |
Formulation — Vitamins
| Component | Concentration (mg/L) |
|---|---|
| Choline chloride | 4.00 |
| D-Calcium pantothenate | 4.00 |
| Folic acid | 4.00 |
| myo-Inositol | 7.20 |
| Nicotinamide | 4.00 |
| Pyridoxal HCl | 4.00 |
| Riboflavin | 0.40 |
| Thiamine HCl | 4.00 |
Formulation — Inorganic Salts
| Component | Concentration (mg/L) |
|---|---|
| CaCl2·2H2O | 265.00 |
| Fe(NO3)3·9H2O | 0.10 |
| KCl | 400.00 |
| MgSO4·7H2O | 200.10 |
| NaCl | 6,400.00 |
| NaHCO3 | 3,700.00 |
| NaH2PO4·2H2O | 141.73 |
Formulation — Other Components
| Component | Concentration (mg/L) |
|---|---|
| D-Glucose | 4,500.00 |
| Phenol red sodium salt | 15.90 |
| Sodium pyruvate | 110.00 |
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