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> Neurofilament (NF-H) (Neuronal Marker) (rNF421), 1mg/mL

Neurofilament (NF-H) (Neuronal Marker) (rNF421), 1mg/mL

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Cat# BNUM2348-50

Size : 50uL

Brand : Biotium

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Neurofilament (NF-H) (Neuronal Marker) (rNF421), 1mg/mL

Neurofilament Recombinant Monoclonal Mouse Antibody (rNF421)

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Formalin-fixed paraffin-embedded human Brain stained with Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).Formalin-fixed paraffin-embedded human Brain stained with Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).
SDS-PAGE Analysis Purified Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421). Confirmation of Purity and Integrity of Antibody.SDS-PAGE Analysis Purified Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Brain tissue lysate using Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).Western Blot Analysis of Human Brain tissue lysate using Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).
  1. Formalin-fixed paraffin-embedded human Brain stained with Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).
  2. SDS-PAGE Analysis Purified Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421). Confirmation of Purity and Integrity of Antibody.
  3. Western Blot Analysis of Human Brain tissue lysate using Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).
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Product Description

This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 2348, Anti-Neurofilament (rNF421) 

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC74CF®740742/767633-685775/50CF®740 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free		N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
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Primary & Secondary Antibody Conjugates

Product Attributes

Antibody number
#2348
Size
50 uL, 100 uL, 250 uL, 500 uL
Antibody reactivity (target)
Neurofilament
Antibody type
Primary
Host species
Mouse
Clonality
Monoclonal, Recombinant Monoclonal
Clone
rNF421
Isotype
IgG1, kappa
Molecular weight
200 kDa
Synonyms
NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200
Human gene symbol
NEFH
Entrez gene ID
4744
SwissProt
P12036
Unigene
19876
Immunogen
Recombinant full-length human NEFH protein
Antibody target cellular localization
Cytoskeleton
Verified antibody applications
Flow (intracellular) (verified), IHC (FFPE) (verified), WB (verified)
Species reactivity
Chicken, Human, Mouse, Pig, Rat
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology (formalin): 0.5-1.0 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Western blotting 1-2 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control
Brain, Neuroblastoma
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas
Cancer, Cytoskeleton, Neuroscience
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression
Neuroendocrine cells, Neurons
Tumor expression
Lung cancer, Neuroendocrine cancer

Documents, Protocols, SDS and COA

Datasheet
Datasheet Download
COA
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