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Reagents and instruments for immunology, cell biology and molecular biology.

Nitazoxanide [55981-09-4]

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Cat# HY-B0217-1ml

Size : 10mM/1mL

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Phone : +1 850 650 7790

Brand : MedChemExpress

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Description

Nitazoxanide (NTZ), an anthelmintic agent, exhibits a broad spectrum of activities against a wide variety of helminths, protozoa, and enteric bacteria infecting animals and humans. Nitazoxanide inhibits Giardia lamblia trophozoite proliferation in axenic culture with an IC₅₀ of 2.4 μM^[1]. Nitazoxanide can be used for the research of parasitic gastroenteritis. Nitazoxanide shows anti-Japanese encephalitis virus (JEV) activity in a mouse model^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 50 μM Compound: NTZ	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
BHK-21	CC₅₀	25 μM Compound: 105	Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay	[PMID: 28689975]
Caco-2	IC₅₀	221 μM Compound: Nitazoxanide	Antiprolferative activity against human Caco2 cells assessed as inhibition of cell proliferation by measuring [3H]-thymidine incorporation incubated for 48 hrs by liquid scintillometry Antiprolferative activity against human Caco2 cells assessed as inhibition of cell proliferation by measuring [3H]-thymidine incorporation incubated for 48 hrs by liquid scintillometry	[PMID: 28601526]
Caco-2	IC₅₀	26.8 μM Compound: NTZ	Antiproliferative activity against human Caco-2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human Caco-2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
HEK-293T	CC₅₀	> 40 μM Compound: Nitazoxanide	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HeLa	IC₅₀	35 μM Compound: NTZ	Cytotoxicity against human HeLa cells assessed as inhibition of cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as inhibition of cell viability measured after 48 hrs by MTT assay	[PMID: 34055214]
HeLa	IC₅₀	35 μM Compound: NTZ	Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
HepG2	CC₅₀	> 40 μM Compound: Nitazoxanide	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HepG2 2.2.15	CC₅₀	> 100 μM Compound: 1, NTZ	Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay	[PMID: 21553812]
HL-60	IC₅₀	20.1 μM Compound: NTZ	Antiproliferative activity against human HL60 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human HL60 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
HT-29	IC₅₀	> 50 μM Compound: NTZ	Antiproliferative activity against human HT29 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human HT29 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
Huh-7	CC₅₀	49 μM Compound: 1, NTZ, PH-5776	Cytotoxicity against human Huh7.5 cells after 3 days by neutral red dye assay Cytotoxicity against human Huh7.5 cells after 3 days by neutral red dye assay	[PMID: 22059983]
PC-3	IC₅₀	44.7 μM Compound: NTZ	Antiproliferative activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
Peritoneal macrophage	IC₅₀	2 mM Compound: 36, NTZ	Anti-inflammatory activity against mouse peritoneal macrophages assessed as reduction in LPS induced IL-6 level by ELISA analysis Anti-inflammatory activity against mouse peritoneal macrophages assessed as reduction in LPS induced IL-6 level by ELISA analysis	[PMID: 32992245]
RAW264.7	IC₅₀	1.54 mM Compound: 36, NTZ	Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in LPS induced IL-6 level by ELISA analysis Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in LPS induced IL-6 level by ELISA analysis	[PMID: 32992245]
U2OS	CC₅₀	25 μM Compound: 105	Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay	[PMID: 28689975]
Vero	IC₅₀	10.74 μM Compound: NTZ	Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay	[PMID: 23787289]
Vero	CC₅₀	833 μM Compound: Nitazoxanide	Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay	[PMID: 25801157]
Vero	CC₅₀	833 μM Compound: NIT, Alinia	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 21397502]
Vero	CC₅₀	833 μM Compound: Nitazoxanide	Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay	[PMID: 28645659]
Vero	CC₅₀	833 μM Compound: NTZ	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay	[PMID: 24529307]
Vero C1008	CC₅₀	> 35.5 μM Compound: 48	Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay	[PMID: 32845145]

In Vitro

Giardia lamblia, a flagellated protozoan, is the most common causative agent of persistent diarrhea worldwide^[1].
Nitazoxanide exhibits effect on G. lamblia trophozoite proliferation in axenic culture with an IC₅₀ of 2.4 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Human cancer colon Caco2 cells were incubated with increasing numbers of Giardia lamblia trophozoites (10³ to 10⁶ parasites per well)
Concentration:	30 μM
Incubation Time:	24 hours
Result:	70 to 90% of the trophozoites remained attached to the Caco2 cells for a period of 24 to 48 h in the absence of Nitazoxanide and at an initial inoculum density of 10⁵ parasites per well. The number of parasites still attached to Caco2 cells after 24 h decreased to less than 20% of the control value in the presence of 30 μM Nitazoxanide with an inoculum density of 10⁵ trophozoites.

In Vivo

Nitazoxanide exhibits a wide spectrum of in vivo activity against a broad spectrum of intestinal parasites, such as Giardia lamblia, Entamoeba histolytica, Trichomonas vaginalis, the apicomplexan Cryptosporidium parvum, and enteric bacteria infecting animals and humans^[1].
Nitazoxanide (50, 75 or 100 mg/kg/day; administered daily by intragastric for up to 25 days) reduces the mortality of Japanese encephalitis virus (JEV) strain-infected mice and protected mice from a lethal dose challenge of JEV^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Three-week old female Chinese Kunming mice (12–14 g body weight) were infected intraperitoneally with JEV^[2]
Dosage:	50, 75 or 100 mg/kg/day
Administration:	Administered intragastrically by gavage
Result:	50 mg/kg/day, 75 mg/kg/day and 100 mg/kg/day led to 30%, 70% and 90% mice survival, respectively.

Molecular Weight

307.28

Formula

C₁₂H₉N₃O₅S

CAS No.

55981-09-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC1=NC=C([N+]([O-])=O)S1)C2=CC=CC=C2OC(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (325.44 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2544 mL	16.2718 mL	32.5436 mL
5 mM	0.6509 mL	3.2544 mL	6.5087 mL
10 mM	0.3254 mL	1.6272 mL	3.2544 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.25 mg/mL (10.58 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

Purity & Documentation

Purity: 99.28%

Data Sheet (281 KB) SDS (393 KB)

COA (253 KB) HNMR (257 KB) RP-HPLC (96 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Rossignol JF, et al. Thiazolides, a new class of anti-influenza molecules targeting viral hemagglutinin at the post-translational level. J Biol Chem. 2009 Oct 23;284(43):29798-808. [Content Brief]

[2]. Zixue Shi, et al. Nitazoxanide inhibits the replication of Japanese encephalitis virus in cultured cells and in a mouse model. Virol J. 2014 Jan 23;11:10. [Content Brief]

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Nitazoxanide [55981-09-4]

Cat# HY-B0217-1ml

Contact local distributor :

Brand : MedChemExpress

Contact local distributor :

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