This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a).