PDC siRNA (Rat)
Cat# MBS829254-15nmol
Size : 15nmol
Brand : MyBiosource
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Phone : +1 850 650 7790
PDC siRNA – Phosducin (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829254MBS829254 is an siRNA targeting PDC. Also known as PDC siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit PDC expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
PDC, siRNA
Full Product Name
PDC siRNA (Rat)
Product Gene Name
PDC sirna
Product Synonym Names
RPR1; Phosducin; PHD; 33 kDa phototransducing protein; Protein MEKA; Rod photoreceptor 1; RPR-1
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
PDC siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
28,129 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PDC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for PDC sirna
RNA Interference (RNAi)
Other Notes
Small volumes of PDC sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for PDC. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosducin
NCBI Official Synonym Full Names
phosducin
NCBI Official Symbol
Pdc
NCBI Official Synonym Symbols
MEKA; Rpr1; 33DPTP
UniProt Protein Name
Phosducin
UniProt Synonym Protein Names
33 kDa phototransducing protein; Protein MEKA; Rod photoreceptor 1; RPR-1
Protein Family
Phosducin
UniProt Gene Name
Pdc
UniProt Synonym Gene Names
Rpr1; PHD; RPR-1
UniProt Entry Name
PHOS_RAT
NCBI Protein Information
phosducin
NCBI Summary for PDC
binds the retinal G-protein transducin; plays a role in negative regulation of phototransduction [RGD, Feb 2006]
UniProt Comments for PDC
phosducin: May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX. Belongs to the phosducin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein regulator, misc.
Cellular Component: photoreceptor inner segment; photoreceptor outer segment; nucleus; cytosol
Biological Process: visual perception
Show more Protein type: G protein regulator, misc.
Cellular Component: photoreceptor inner segment; photoreceptor outer segment; nucleus; cytosol
Biological Process: visual perception
Research Articles on PDC
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with PDC sirna
| Reference Product | PubMed Publications |
|---|---|
| Rho sirna | >7 publications with PDC and Rho |
| Ywhaz sirna | >1 publications with PDC and Ywhaz |
Pathways associated with PDC sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Olfactory Transduction Pathway antibodies | Olfactory Transduction Pathway Diagram |
| Olfactory Transduction Pathway antibodies | Olfactory Transduction Pathway Diagram |
Diseases associated with PDC sirna
| Disease Name | Pubmed Publications |
|---|---|
| Liver Diseases Antibodies | >326 publications with PDC and Liver Diseases |
| Necrosis Antibodies | >161 publications with PDC and Necrosis |
| Carcinoma Antibodies | >142 publications with PDC and Carcinoma |
| Vascular Diseases Antibodies | >116 publications with PDC and Vascular Diseases |
| Heart Diseases Antibodies | >85 publications with PDC and Heart Diseases |
| Neoplasms, Experimental Antibodies | >43 publications with PDC and Neoplasms, Experimental |
| Neurobehavioral Manifestations Antibodies | >36 publications with PDC and Neurobehavioral Manifestations |
| Neoplasm Metastasis Antibodies | >35 publications with PDC and Neoplasm Metastasis |
| Atrophy Antibodies | >31 publications with PDC and Atrophy |
| Diabetes Mellitus, Type 2 Antibodies | >22 publications with PDC and Diabetes Mellitus, Type 2 |
Organs/Tissues associated with PDC sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Eye Antibodies | >56 publications with PDC and Eye |