Phospho-Dnmt1 (Ser714) Antibody
Cat# MBS9612760-0,1mL
Size : 0.1mL
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Western blot analysis of extracts from Mouse brain, using Phospho-Dnmt1 (Ser714) Antibody. The lane on the left was treated with blocking peptide.
Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
At 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
The nuclear counter stain is DAPI (blue).
The nuclear counter stain is DAPI (blue).
DNMT1 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9612760MBS9612760 is an antibody targeting Dnmt1. Also known as Phospho-Dnmt1 (Ser714) Antibody. Reactive in Human, Mouse. Host: Rabbit. More details
Product Overview
Online Datasheet in PDFProduct Name
Dnmt1, Polyclonal Antibody
Full Product Name
Phospho-Dnmt1 (Ser714) Antibody
Product Gene Name
anti-Dnmt1 antibody
Product Synonym Gene Name
DNMT1
Product Synonym Names
ADCADN; AIM; CXXC finger protein 9; CXXC-type zinc finger protein 9; CXXC9; DNA (cytosine 5) methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase M.HsaI.; DNA MTase; DNA MTase HsaI; DNMT 1; DNMT; Dnmt1; DNMT1_HUMAN; Dnmt1o; FLJ16293; HSN1E; M.HsaI; MCMT; Met1; MGC104992; mMmul; MommeD2
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Peptide affinity purification
Concentration
~1mg/ml (lot specific)
Specificity
Phospho-Dnmt1 (Ser714) Antibody detects endogenous levels of Dnmt1 only when phosphorylated at Ser714.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Dnmt1 (Ser714) peptide (MBS9616041) is used for blocking the activity of Dnmt1 (Ser714) antibody (MBS9612760)
OMIM
phenotype 614116
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Dnmt1 around the phosphorylation site of Ser714.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-Dnmt1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Application Notes for anti-Dnmt1 antibody
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
*The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
*The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (IHC) of anti-Dnmt1 antibody
Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Immunohistochemistry (IHC) of anti-Dnmt1 antibody
At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Immunohistochemistry (IHC) of anti-Dnmt1 antibody
At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Immunohistochemistry (IHC) of anti-Dnmt1 antibody
At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Immunohistochemistry (IHC) of anti-Dnmt1 antibody
At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Immunohistochemistry (IHC) of anti-Dnmt1 antibody
At 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Testing Data of anti-Dnmt1 antibody
At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
The nuclear counter stain is DAPI (blue).
The nuclear counter stain is DAPI (blue).
Other Notes
Small volumes of anti-Dnmt1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for Dnmt1. It may not necessarily be applicable to this product.
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 1
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 1
NCBI Official Symbol
DNMT1
NCBI Official Synonym Symbols
AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Synonym Protein Names
CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
Protein Family
DNA
UniProt Gene Name
DNMT1
UniProt Synonym Gene Names
AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
UniProt Entry Name
DNMT1_HUMAN
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9
NCBI Summary for Dnmt1
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Show more UniProt Comments for Dnmt1
DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.
Protein type: Methyltransferase, DNA; EC 2.1.1.37; Cell development/differentiation; Methyltransferase; Transcription regulation; Amino Acid Metabolism - cysteine and methionine
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus
Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; DNA-methyltransferase activity; transcription factor binding
Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; chromatin modification; gene silencing; regulation of gene expression, epigenetic; DNA methylation on cytosine; regulation of cell proliferation
Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
Show more Protein type: Methyltransferase, DNA; EC 2.1.1.37; Cell development/differentiation; Methyltransferase; Transcription regulation; Amino Acid Metabolism - cysteine and methionine
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus
Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; DNA-methyltransferase activity; transcription factor binding
Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; chromatin modification; gene silencing; regulation of gene expression, epigenetic; DNA methylation on cytosine; regulation of cell proliferation
Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
Research Articles on Dnmt1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-Dnmt1 antibody
| Reference Product | PubMed Publications |
|---|---|
| DNMT3B antibody | >506 publications with Dnmt1 and DNMT3B |
| MECP2 antibody | >100 publications with Dnmt1 and MECP2 |
| HDAC1 antibody | >99 publications with Dnmt1 and HDAC1 |
| UHRF1 antibody | >67 publications with Dnmt1 and UHRF1 |
| PCNA antibody | >65 publications with Dnmt1 and PCNA |
| DMAP1 antibody | >63 publications with Dnmt1 and DMAP1 |
| SUV39H1 antibody | >30 publications with Dnmt1 and SUV39H1 |
| RB1 antibody | >7 publications with Dnmt1 and RB1 |
| USP7 antibody | >6 publications with Dnmt1 and USP7 |
Pathways associated with anti-Dnmt1 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| DNA Methylation Pathway antibodies | DNA Methylation Pathway Diagram |
| Epigenetic Regulation Of Gene Expression Pathway antibodies | Epigenetic Regulation Of Gene Expression Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Metabolic Pathways antibodies | Metabolic Pathways Diagram |
| Methionine Degradation Pathway antibodies | Methionine Degradation Pathway Diagram |
| Methionine Degradation Pathway antibodies | Methionine Degradation Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
Diseases associated with anti-Dnmt1 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Carcinoma Antibodies | >218 publications with Dnmt1 and Carcinoma |
| Adenocarcinoma Antibodies | >100 publications with Dnmt1 and Adenocarcinoma |
| Breast Neoplasms Antibodies | >87 publications with Dnmt1 and Breast Neoplasms |
| Cell Transformation, Neoplastic Antibodies | >61 publications with Dnmt1 and Cell Transformation, Neoplastic |
| Disease Models, Animal Antibodies | >58 publications with Dnmt1 and Disease Models, Animal |
| Liver Diseases Antibodies | >58 publications with Dnmt1 and Liver Diseases |
| Lung Neoplasms Antibodies | >57 publications with Dnmt1 and Lung Neoplasms |
| Liver Neoplasms Antibodies | >51 publications with Dnmt1 and Liver Neoplasms |
| Carcinoma, Hepatocellular Antibodies | >50 publications with Dnmt1 and Carcinoma, Hepatocellular |
| Neoplasms, Experimental Antibodies | >40 publications with Dnmt1 and Neoplasms, Experimental |
Organs/Tissues associated with anti-Dnmt1 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >173 publications with Dnmt1 and Blood |
| Brain Antibodies | >129 publications with Dnmt1 and Brain |
| Liver Antibodies | >115 publications with Dnmt1 and Liver |
| Lung Antibodies | >95 publications with Dnmt1 and Lung |
| Embryonic Tissue Antibodies | >47 publications with Dnmt1 and Embryonic Tissue |
| Prostate Antibodies | >46 publications with Dnmt1 and Prostate |
| Stomach Antibodies | >39 publications with Dnmt1 and Stomach |
| Nerve Antibodies | >34 publications with Dnmt1 and Nerve |
| Bone Antibodies | >33 publications with Dnmt1 and Bone |
| Muscle Antibodies | >33 publications with Dnmt1 and Muscle |