Polyclonal Goat Anti-Rat IgG2b Secondary Antibody (FITC, IHC, IF)

Rat IgG2b

Goat

Rat (tested or 100% immunogen sequence identity)

Polyclonal

FITC. Also available conjugated with HRP, TRITC.

Purified IgG

Unmodified

Pools of purified homogenous IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat IgG2b, subclass specific. Cross-reactivity of this product has not been tested in detail; however, in double radial immunodiffusion a weak reaction with Mouse has been observed.

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the subclass IgG2b as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Further tested on the level of sensitivity of direct and indirect immunofluorescence staining methods applied to lymphoid cells. When used on vital peripheral blood cells this immunoconjugate does not react with Fc receptors present on cell surface membranes. To identify and measure IgG2b, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.