Reagents and instruments for immunology, cell biology and molecular biology.
 
> PRKDC, phosphorylated (Thr2609) (DNA-dependent Protein Kinase Catalytic Subunit, DNA-PK Catalytic Subunit, DNA-PKcs, DNPK1, p460, HYRC, HYRC1)

PRKDC, phosphorylated (Thr2609) (DNA-dependent Protein Kinase Catalytic Subunit, DNA-PK Catalytic Subunit, DNA-PKcs, DNPK1, p460, HYRC, HYRC1)

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Cat# P9003-01C-200ul

Size : 200ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

P9003-01C PRKDC, phosphorylated (Thr2609) (DNA-dependent Protein Kinase Catalytic Subunit, DNA-PK Catalytic Subunit, DNA-PKcs, DNPK1, p460, HYRC, HYRC1)

PRKDC, phosphorylated
Dot blot analysis of anti-DNA-PK-pT2609 Pab (RB08113) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Clone Type
Polyclonal
Host
rabbit
Source
human
Swiss Prot
P78527
Isotype
IgG
Grade
Affinity Purified
Applications
DB E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Stem cell surface receptors and activate yeast MAPK pathway.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: Not Determined |Form: Supplied as a liquid in PBS, 0.09% sodium azide.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr2609 of human DNA-PK (KLH).|Specificity: Recognizes human DNA-PK when phosphorylated at Thr2609.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr2609 of human DNA-PK (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human DNA-PK when phosphorylated at Thr2609.
References
1.Goudelock, D.M., et al., J. Biol. Chem. 278(32):29940-29947 (2003). 2.Ding, Q., et al., Mol. Cell. Biol. 23(16):5836-5848 (2003). 3.Lucero, H., et al., J. Biol. Chem. 278(24):22136-22143 (2003). 4.Calsou, P., et al., J. Mol. Biol. 326(1):93-103 (2003). 5.Karpova, A.Y., et al., Proc. Natl. Acad. Sci. USA 99(5):2818-2823 (2002)