Propidium Iodide Solution

The PI (Propidium iodide) solution can be used to exclude non-viable cells in flow cytometry assays. PI is a fluorescent dye that binds to double stranded DNA in cells that have lost plasma membrane integrity, such as those in the late stages of apoptosis or dead cells. It is detected in the orange 562-588 nm band-pass filter.

Martin, S., Reutelingsperger, C. P., McGahon, A. J., Rader, J. A., Van Schie, R. C., LaFace, D. M., & Green, D. R. (1995). Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. The Journal of experimental medicine, 182(5), 1545-1556.
Douglas, R. S., Tarshis, A. D., Pletcher Jr, C. H., Nowell, P. C., & Moore, J. S. (1995). A simplified method for the coordinate examination of apoptosis and surface phenotype of murine lymphocytes. Journal of immunological methods,188(2), 219-228.

Small volumes of Propidium Iodide Solution accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS697171 is an accessory reagent targeting Propidium Iodide Solution.