C0001-1mL | Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)

Ingredient	CAS	MW	Concentration (100x)	Target	Type
AEBSF	30827-99-7	239.69	104 mM	serine proteases	Irreversible
Aprotinin	9087-70-1	6511.44	80 μM	serine proteases	Reversible
Bestatin	58970-76-6	308.37	5 mM	aminopeptidase B; LAP	Reversible
E-64	66701-25-5	357.41	1.5 mM	cysteine proteases	Irreversible
Leupeptin	55123-66-5	475.6	2 mM	cysteine proteases; serine proteases	Reversible
Pepstatin A	26305-03-3	685.89	1.5 mM	aspartic proteinase	Reversible

Accurate Ingredients —— Accurate components and clear concentrations to avoid experimental uncertainty
Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
Cost-Effective —— Lower price with higher quality

Compatible with Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Thaw in Room Temperature. Please centrifuge it at low speed before opening the cover, so as to throw the liquid adhered to the wall of the tube to the bottom.
Add concentrated cocktail at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Briefly vortex cocktails to help facilitate the dissolution.
Extracts of up to 20 g wet weight.

Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
Some DUB proteases (one example is ATAXIN-3) cannot be suppressed by traditional protease inhibitors, such as E-64, AEBSF, bestatin, leupeptin and Aprotinin.

(Reference: Neil D. Rawlings, Guy Salvesen et al. Handbook of Proteolytic Enzymes, Vol.1, 2012.)

Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)