Proteinase 3, Human Peripheral Blood Leukocytes (cANCA, PRTN3)

Proteinase 3 is one of the serine proteinases localized in azurophil granules of polymorphonuclear leukocytes. It has been identified as the autoantigen of Wegener granulomatosis, a systemic vasculitis complicated by melomonocyte proliferation. It exhibits proteolytic activity towards a range of substrates. It is physiologically inhibited by alpha1-antitrypsin. The protein has been described as having antibiotic and growth-promoting properties and causing emphysema when administered by tracheal insufflation. PRTN3 degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema once managed by tracheal insufflation to hamsters.

Source:
Proteinase 3 from Human peripheral blood leukocytes

Synonyms:
PRTN3; AGP7; P29; PR-3; ACPA; C-ANCA; MBT; MBN; Leukocyte proteinase 3; Neutrophil proteinase 4; Wegener granulomatosis autoantigen; Azurophil granule protein 7; myeloblastin; Serine proteinase neutrophil

Molecular Weight:
25kD

Appearance:
Supplied as a liquid in 20mM Sodium Phosphate pH-6.2, 300mM NaCl, 0.02% Lubrol.

Purity:
~98% (SDS-PAGE)

Applications:
Suitable for use in ELISA.
ELISA: Coating concentration: 0.5-1.0ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.