Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections: Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Reactivity
Rat
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
PHA stimulated rat lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Specificity
This antibody recognises CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like).
The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
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