Rabbit Anti-ACTB Polyclonal Antibody

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Size : 100ul

Phone : +1 850 650 7790

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Phone : +1 850 650 7790

HPA041264

actin, beta

Anti-ACTB Antibody

Polyclonal Antibody against HUMAN ACTB

0.1 mg/ml

info@biovalley.fr

Immunohistochemical staining of human tonsil shows strong positivity in lymphoid cells.

Target protein: actin, beta
Target gene: ACTB
Verified species reactivity: Human, Mouse, Rat
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000062825 (100%)RAT - ENSRNOG00000034254 (100%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Immunohistochemistry (IHC)
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Human Protein Atlas