Rabbit Anti-DNMT3B Polyclonal Antibody

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Cat# HPA001595

Size : 100ul

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Phone : +1 850 650 7790

Brand : Atlas Antibodies

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Contact local distributor :

Phone : +1 850 650 7790

HPA001595

DNA (cytosine-5-)-methyltransferase 3 beta

Anti-DNMT3B Antibody

Polyclonal Antibody against HUMAN DNMT3B

0.1 mg/ml

info@biovalley.fr

Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA001595 antibody. Corresponding DNMT3B RNA-seq data are presented for the same tissues.

Product details

Target protein: DNA (cytosine-5-)-methyltransferase 3 beta
Target gene: DNMT3B
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000027478 (62%)RAT - ENSRNOG00000010625 (66%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000050731
Concentration: 0.1 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:200 - 1:500
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Western Blot (WB)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Target information

Protein name: DNA (cytosine-5-)-methyltransferase 3 beta
Gene name: DNMT3B
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (11)

Shokry D, Khan MW, Powell C, Johnson S, Rennels BC, Boyd RI, Sun Z, Fazal Z, Freemantle SJ, Parker MH, Vieson MD, Samuelson JP, Spinella MJ, Singh RCell Commun Signal , 2024 Oct 31; 22:528. Epub 2024 Oct 182024 Oct 31
Gondaliya P, Jash K, Srivastava A, Kalia KJ Diabetes Metab Disord , 2023 May 25; 22(2):1105-1115. Epub 2023 May 252023 May 25