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> Rabbit Anti-DNMT3L Polyclonal Antibody

Rabbit Anti-DNMT3L Polyclonal Antibody

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Cat# HPA055234

Size : 100ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA055234
DNA (cytosine-5-)-methyltransferase 3-like

Anti-DNMT3L Antibody

Polyclonal Antibody against HUMAN DNMT3L

0.05 mg/ml
Anti-DNMT3L Antibody
Anti-DNMT3L Antibody
Anti-DNMT3L Antibody
Anti-DNMT3L Antibody
Anti-DNMT3L Antibody
info@biovalley.fr

Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Product details

  • Target protein: DNA (cytosine-5-)-methyltransferase 3-like
  • Target gene: DNMT3L
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000000730 (63%)RAT - ENSRNOG00000001212 (66%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: R73467
  • Concentration: 0.05 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: DNA (cytosine-5-)-methyltransferase 3-like
  • Gene name: DNMT3L
  • Alternative gene names: MGC1090, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (1)

  • This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
    Human Protein Atlas