Rabbit Anti-MAP1S (Center) Polyclonal Antibody
Cat# AP5769c-ev
Size : 80ul
Brand : Abcepta
Contact local distributor :
Phone : +1 850 650 7790
MAP1S Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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MAP1S Antibody (Center) (Cat. #AP5769c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MAP1S antibody detected the MAP1S protein (arrow).
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MAP1S Antibody (Center) (Cat. #AP5769c) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAP1S Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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MAP1S Antibody (Center) (Cat. #AP5769c) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MAP1S Antibody (Center) (Cat. #AP5769c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MAP1S antibody detected the MAP1S protein (arrow).
Application 
| FC, WB, IHC-P, E |
|---|---|
| Primary Accession | Q66K74 |
| Other Accession | NP_060644.4 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 112211 Da |
| Antigen Region | 493-520 aa |
| Gene ID | 55201 |
|---|---|
| Other Names | Microtubule-associated protein 1S, MAP-1S, BPY2-interacting protein 1, Microtubule-associated protein 8, Variable charge Y chromosome 2-interacting protein 1, VCY2-interacting protein 1, VCY2IP-1, MAP1S heavy chain, MAP1S light chain, MAP1S, BPY2IP1, C19orf5, MAP8, VCY2IP1 |
| Target/Specificity | This MAP1S antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 493-520 amino acids from the Central region of human MAP1S. |
| Dilution | FC~~1:10~50 WB~~1:1000 IHC-P~~1:50~100 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MAP1S Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | MAP1S |
|---|---|
| Synonyms | BPY2IP1, C19orf5, MAP8, VCY2IP1 |
| Function | Microtubule-associated protein that mediates aggregation of mitochondria resulting in cell death and genomic destruction (MAGD). Plays a role in anchoring the microtubule organizing center to the centrosomes. Binds to DNA. Plays a role in apoptosis. Involved in the formation of microtubule bundles (By similarity). |
| Cellular Location | Nucleus. Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Note=Detected in filopodia-like protrusions and synapses (By similarity). Detected in perinuclear punctate network corresponding to mitochondrial aggregates and in the nucleus in cells exhibiting apoptosis. Associated specifically with microtubules stabilized by paclitaxel and colocalizes with RASSF1 isoform A. In interphase cells, shows a diffuse cytoplasmic staining with partial localization to the microtubules. During the different stages of mitosis detected at the spindle microtubules. |
| Tissue Location | Expressed in neurons (at protein level). Expressed in spermatocytes, spermatids and spermatozoa. Expressed in the cerebral cortex. Highly expressed in testis. Moderately expressed in the brain, colon, heart, kidney, liver, lung, placenta, small intestine, spleen and stomach. Weakly expressed in muscle. |
| Author : Bai W1,Bai J2,Li Y3,Tian D3,Shi R4. Biochem Biophys Res Commun. 2017 Feb 7. pii: S0006-291X(17)30302-9. doi: 10.1016/j.bbrc.2017.02.034. [Epub ahead of print] Application: WB | 28188784 |
Provided below are standard protocols that you may find useful for product applications.
Background
Microtubule-associated protein that mediates aggregation of mitochondria resulting in cell death and genomic destruction (MAGD). Plays a role in anchoring the microtubule-organizing center to the centrosomes. Binds to DNA. Plays a role in apoptosis. Involved in the formation of microtubule bundles (By similarity).