Rabbit Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit
Cat# MBS2023231-96
Size : 96-Strip-Wells
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit for Rabbit
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2023231SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). Standards or samples are then added to the appropriate… More details
Product Overview
Online Datasheet in PDFProduct Name
Fibroblast Growth Factor 2, Basic (FGF2), ELISA Kit
Full Product Name
Rabbit Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit
Product Gene Name
FGF2 elisa kit
Product Synonym Names
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
Species Reactivity
Rabbit
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
5.9pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2).
No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Molecular Weight
21,203 Da
OMIM
134920
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Product Categories/Family for FGF2 elisa kit
Cytokine; Tumor immunity; Infection immunity
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of FGF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for FGF2 purchase
MBS2023231 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FGF2. The ELISA analytical biochemical technique of the MBS2023231 kit is based on FGF2 antibody-FGF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FGF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FGF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for FGF2. It may not necessarily be applicable to this product.
NCBI Official Full Name
fibroblast growth factor 2
NCBI Official Synonym Full Names
fibroblast growth factor 2 (basic)
NCBI Official Symbol
FGF2
NCBI Official Synonym Symbols
BFGF; FGFB; FGF-2; HBGF-2
UniProt Protein Name
Fibroblast growth factor 2
UniProt Synonym Protein Names
Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
Protein Family
Fibroblast growth factor
UniProt Gene Name
FGF2
UniProt Synonym Gene Names
FGFB; FGF-2; bFGF; HBGF-2
UniProt Entry Name
FGF2_HUMAN
NCBI Protein Information
fibroblast growth factor 2
NCBI Summary for FGF2
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for FGF2
FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.
Protein type: Activator; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q26
Cellular Component: extracellular space; extracellular region; nucleus
Molecular Function: heparin binding; protein binding; growth factor activity; ligand-dependent nuclear receptor transcription coactivator activity; cytokine activity; fibroblast growth factor receptor binding; chemoattractant activity
Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; activation of MAPK activity; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; chemotaxis; signal transduction; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; organ morphogenesis; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; release of sequestered calcium ion into cytosol; positive regulation of cell division; Ras protein signal transduction; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; inositol phosphate biosynthetic process
Show more Protein type: Activator; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q26
Cellular Component: extracellular space; extracellular region; nucleus
Molecular Function: heparin binding; protein binding; growth factor activity; ligand-dependent nuclear receptor transcription coactivator activity; cytokine activity; fibroblast growth factor receptor binding; chemoattractant activity
Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; activation of MAPK activity; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; chemotaxis; signal transduction; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; organ morphogenesis; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; release of sequestered calcium ion into cytosol; positive regulation of cell division; Ras protein signal transduction; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; inositol phosphate biosynthetic process
Research Articles on FGF2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with FGF2 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Activated Point Mutants Of FGFR2 Pathway antibodies | Activated Point Mutants Of FGFR2 Pathway Diagram |
| Adaptive Immune System Pathway antibodies | Adaptive Immune System Pathway Diagram |
| Angiogenesis Pathway antibodies | Angiogenesis Pathway Diagram |
| Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies | Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram |
| Cardiac Progenitor Differentiation Pathway antibodies | Cardiac Progenitor Differentiation Pathway Diagram |
| Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies | Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram |
| DAP12 Interactions Pathway antibodies | DAP12 Interactions Pathway Diagram |
| DAP12 Signaling Pathway antibodies | DAP12 Signaling Pathway Diagram |
| Developmental Biology Pathway antibodies | Developmental Biology Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
Diseases associated with FGF2 elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >981 publications with FGF2 and Nervous System Diseases |
| Necrosis Antibodies | >617 publications with FGF2 and Necrosis |
| Wounds and Injuries Antibodies | >571 publications with FGF2 and Wounds and Injuries |
| Inflammation Antibodies | >443 publications with FGF2 and Inflammation |
| Heart Diseases Antibodies | >419 publications with FGF2 and Heart Diseases |
| Myocardial Ischemia Antibodies | >347 publications with FGF2 and Myocardial Ischemia |
| Disease Progression Antibodies | >339 publications with FGF2 and Disease Progression |
| Lung Diseases Antibodies | >321 publications with FGF2 and Lung Diseases |
| Hyperplasia Antibodies | >258 publications with FGF2 and Hyperplasia |
| Glioma Antibodies | >226 publications with FGF2 and Glioma |
Organs/Tissues associated with FGF2 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Vascular Antibodies | >4706 publications with FGF2 and Vascular |
| Brain Antibodies | >2178 publications with FGF2 and Brain |
| Bone Antibodies | >1811 publications with FGF2 and Bone |
| Muscle Antibodies | >1797 publications with FGF2 and Muscle |
| Embryonic Tissue Antibodies | >1268 publications with FGF2 and Embryonic Tissue |
| Eye Antibodies | >1023 publications with FGF2 and Eye |
| Skin Antibodies | >822 publications with FGF2 and Skin |
| Connective Tissue Antibodies | >707 publications with FGF2 and Connective Tissue |
| Bone Marrow Antibodies | >652 publications with FGF2 and Bone Marrow |
| Heart Antibodies | >650 publications with FGF2 and Heart |