Rabbit Polyclonal to Human EDNRA / Endothelin A Receptor
Cat# MBS243217-0,05mg
Size : 0.05mg
Brand : MyBiosource
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Anti-Endothelin A Receptor antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
EDNRA Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243217The ETA receptor is an Endothelin Receptor that mediates the effects of endothelin-1, a mitogen with potent contractile properties. In the human fetal olfactory epithelium cell line FNC-B4, the ETA receptor increases the secretion of gonadotropin-releasing… More details
Product Overview
Online Datasheet in PDFProduct Name
EDNRA / Endothelin A Receptor, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human EDNRA / Endothelin A Receptor
Product Gene Name
anti-EDNRA antibody
Product Synonym Names
Anti-EDNRA / Endothelin A Receptor Antibody (Extracellular Domain) IHC-plus; EDNRA; Endothelin receptor subtype A; Et-a-r; ETRA; Endothelin A receptor; Endothelin eta receptor; Endothelin type a receptor; Endothelin-1 receptor; ETAR; Endothelin receptor type A; HET-AR; Et-a receptor; ETA; ETA-R; Human EDNRA; Endothelin A Receptor
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey
Predicted Reactivity: Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit
Predicted Reactivity: Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit
Form/Format
PBS, 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human Endothelin A Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except EDNRB (72%).
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
OMIM
131243
Family/Subfamily
GPCR / Endothelin
Immunogen Species
EDNRA / Endothelin A Receptor antibody was raised against Human
Immunogen Type
Synthetic peptide
Immunogen
EDNRA / Endothelin A Receptor antibody was raised against synthetic 18 amino acid peptide from 3rd extracellular domain of human Endothelin A Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit, Horse, Pig (94%); Sheep, Bovine, Bat, Opossum, Platypus, Medaka (89%).
Epitope
Extracellular Domain
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:
Tissue Preparation:
Formalin fixation and embedding in paraffin wax.
Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.
Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT
Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT
Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT
Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT
Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Tissue Preparation:
Formalin fixation and embedding in paraffin wax.
Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.
Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT
Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT
Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT
Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT
Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Product Categories/Family for anti-EDNRA antibody
Family: GPCR; Subfamily: Endothelin
Applications Tested/Suitable for anti-EDNRA antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes for anti-EDNRA antibody
IHC-P (3 - 16 ug/ml)
Other Notes
Small volumes of anti-EDNRA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for EDNRA purchase
MBS243217 is an antibody targeting EDNRA. Also known as Rabbit Polyclonal to Human EDNRA / Endothelin A Receptor. Reactive in Human, Monkey
Predicted Reactivity: Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit. Host: Rabbit.
Predicted Reactivity: Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for EDNRA. It may not necessarily be applicable to this product.
NCBI Official Full Name
endothelin-1 receptor isoform a
NCBI Official Synonym Full Names
endothelin receptor type A
NCBI Official Symbol
EDNRA
NCBI Official Synonym Symbols
ETA; ET-A; ETAR; ETRA; ETA-R; hET-AR
UniProt Protein Name
Endothelin-1 receptor
UniProt Synonym Protein Names
Endothelin A receptor; ET-A; ETA-R; hET-AR
Protein Family
Endothelin-1 receptor
UniProt Gene Name
EDNRA
UniProt Synonym Gene Names
ETA; ETRA; ET-A; ETA-R; hET-AR
UniProt Entry Name
EDNRA_HUMAN
NCBI Protein Information
endothelin-1 receptor; G protein-coupled receptor; endothelin receptor subtype A; endothelin-1-specific receptor
NCBI Summary for EDNRA
This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Show more UniProt Comments for EDNRA
EDNRA: Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: GPCR, family 1; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR
Chromosomal Location of Human Ortholog: 4q31.22
Cellular Component: nuclear membrane; integral to plasma membrane; T-tubule; plasma membrane; lipid raft
Molecular Function: endothelin receptor activity; protein binding; phosphoinositide phospholipase C activity
Biological Process: positive regulation of odontogenesis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); neural crest cell development; metabolic process; heart development; response to morphine; smooth muscle cell proliferation; response to lipopolysaccharide; glucose transport; signal transduction; sensory perception of pain; enteric nervous system development; regulation of epithelial cell proliferation; Rho protein signal transduction; histamine secretion; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; smooth muscle contraction; fibroblast proliferation; regulation of blood pressure; positive regulation of cell proliferation; protein kinase C activation; artery smooth muscle contraction; penile erection; aging; vasoconstriction; in utero embryonic development; adenylate cyclase activation; glomerular filtration; respiratory gaseous exchange; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; cell proliferation; phospholipase C activation; maternal process involved in parturition; response to hypoxia; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of inflammatory response; negative regulation of apoptosis
Disease: Mandibulofacial Dysostosis With Alopecia; Migraine With Or Without Aura, Susceptibility To, 1
Show more Protein type: GPCR, family 1; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR
Chromosomal Location of Human Ortholog: 4q31.22
Cellular Component: nuclear membrane; integral to plasma membrane; T-tubule; plasma membrane; lipid raft
Molecular Function: endothelin receptor activity; protein binding; phosphoinositide phospholipase C activity
Biological Process: positive regulation of odontogenesis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); neural crest cell development; metabolic process; heart development; response to morphine; smooth muscle cell proliferation; response to lipopolysaccharide; glucose transport; signal transduction; sensory perception of pain; enteric nervous system development; regulation of epithelial cell proliferation; Rho protein signal transduction; histamine secretion; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; smooth muscle contraction; fibroblast proliferation; regulation of blood pressure; positive regulation of cell proliferation; protein kinase C activation; artery smooth muscle contraction; penile erection; aging; vasoconstriction; in utero embryonic development; adenylate cyclase activation; glomerular filtration; respiratory gaseous exchange; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; cell proliferation; phospholipase C activation; maternal process involved in parturition; response to hypoxia; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of inflammatory response; negative regulation of apoptosis
Disease: Mandibulofacial Dysostosis With Alopecia; Migraine With Or Without Aura, Susceptibility To, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-EDNRA antibody
| Reference Product | PubMed Publications |
|---|---|
| EDN1 antibody | >48 publications with EDNRA and EDN1 |
| EDN2 antibody | >12 publications with EDNRA and EDN2 |
| EDN3 antibody | >8 publications with EDNRA and EDN3 |
| NTS antibody | >1 publications with EDNRA and NTS |
Pathways associated with anti-EDNRA antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Calcium Signaling Pathway antibodies | Calcium Signaling Pathway Diagram |
| Calcium Signaling Pathway antibodies | Calcium Signaling Pathway Diagram |
| Class A/1 (Rhodopsin-like Receptors) Pathway antibodies | Class A/1 (Rhodopsin-like Receptors) Pathway Diagram |
| Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies | Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
| EGFR-dependent Endothelin Signaling Events Pathway antibodies | EGFR-dependent Endothelin Signaling Events Pathway Diagram |
| Endothelin Pathway antibodies | Endothelin Pathway Diagram |
| Endothelins Pathway antibodies | Endothelins Pathway Diagram |
| G Alpha (q) Signalling Events Pathway antibodies | G Alpha (q) Signalling Events Pathway Diagram |
| GPCR Downstream Signaling Pathway antibodies | GPCR Downstream Signaling Pathway Diagram |
Diseases associated with anti-EDNRA antibody
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >19 publications with EDNRA and Nervous System Diseases |
| Cardiovascular Diseases Antibodies | >18 publications with EDNRA and Cardiovascular Diseases |
| Hypertension Antibodies | >15 publications with EDNRA and Hypertension |
| Lung Diseases Antibodies | >11 publications with EDNRA and Lung Diseases |
| Craniofacial Abnormalities Antibodies | >10 publications with EDNRA and Craniofacial Abnormalities |
| Inflammation Antibodies | >7 publications with EDNRA and Inflammation |
| Kidney Diseases Antibodies | >5 publications with EDNRA and Kidney Diseases |
| Hypertension, Pulmonary Antibodies | >5 publications with EDNRA and Hypertension, Pulmonary |
| Heart Diseases Antibodies | >4 publications with EDNRA and Heart Diseases |
| Necrosis Antibodies | >4 publications with EDNRA and Necrosis |
Organs/Tissues associated with anti-EDNRA antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Vascular Antibodies | >31 publications with EDNRA and Vascular |
| Muscle Antibodies | >17 publications with EDNRA and Muscle |
| Embryonic Tissue Antibodies | >16 publications with EDNRA and Embryonic Tissue |
| Bone Antibodies | >13 publications with EDNRA and Bone |
| Lung Antibodies | >12 publications with EDNRA and Lung |
| Kidney Antibodies | >9 publications with EDNRA and Kidney |
| Heart Antibodies | >8 publications with EDNRA and Heart |
| Ovary Antibodies | >7 publications with EDNRA and Ovary |
| Placenta Antibodies | >5 publications with EDNRA and Placenta |
| Eye Antibodies | >5 publications with EDNRA and Eye |
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